Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 377
Objective. To evaluate the differential physiological effects of dietary fiber (MF) and phenolic compounds (MP) isolated from freeze-dried mango cv. "Ataulfo" pulp (FDM) in a non-alcoholic steatohepatitis (NASH) rat model. Methods. Male albino rats (166 g) were fed for twelve weeks with five isoenergetic (3.4 kcal/g) diets (6 rats/diet): control, high cholesterol (0.8%)/ sodium cholate (0.2%) alone (HCC) or supplemented with MF (6 %), MP (0.1 %) or FDM (5 %). Serum lipids and cytokine levels, hepatic histomorphometry, fatty acid/cholesterol deposition and gene expression (PPARα, LXRα, APOA1, and APOB mRNA levels) were evaluated. Results. The MP+HCC group showed a transiently higher APOA1/APOB ratio in serum, but the hepatic gene expression of both proteins was similar among groups; while MF caused a lower serum level of IL-1α/β, IFN-γ, TNF-α, FDM increased IL-4, IL-6 and IL-10 levels. NASH was more pronounced in HCC (alone) group, but all mango-supplemented diets reduced NASH to a different extent (MP>MF>FDM), a fact associated with a lower cholesterol deposition (-33%) and a different fatty acid profile. A higher PPARα and LXRα mRNA level was found in MP and MF-fed groups, respectively. Conclusions: MP and MF exert synergistic hepatoprotective effects in HCC-fed rats; such effects are more evident when supplemented alone than combined (FDM).
Mexican Council of Science and Technology (CONACyT), projects 563 (Un enfoque multidisciplinario de la farmacocinética de polifenoles de
mango Ataulfo: Interacciones ,moleculares, estudios preclínicos y clínicos" y CB-2015-1/254063 (Antioxidantes de frutas y verduras en cáncer, ¿Son su bioaccesibilidad, biodisponibilidad o sinergismo estructural responsables de su ineficacia?*.
Universidad Autonoma de Ciudad Juarez - Instituto de Ciencias Biomedicas
Ciudad Juarez, Chihuahua, Mexico