Poster Topical Area: Dietary Bioactive Components

Location: Hall D

Poster Board Number: 377

P08-119 - Dietary fiber and phenolic compounds from Mango “Ataulfo” pulp exert different yet complementary metabolic effects in rats with non-alcoholic steatohepatitis

Monday, Jun 11
8:00 AM – 3:00 PM

Objective. To evaluate the differential physiological effects of dietary fiber (MF) and phenolic compounds (MP) isolated from freeze-dried mango cv. "Ataulfo" pulp (FDM) in a non-alcoholic steatohepatitis (NASH) rat model. Methods. Male albino rats (166 g) were fed for twelve weeks with five isoenergetic (3.4 kcal/g) diets (6 rats/diet): control, high cholesterol (0.8%)/ sodium cholate (0.2%) alone (HCC) or supplemented with MF (6 %), MP (0.1 %) or FDM (5 %). Serum lipids and cytokine levels, hepatic histomorphometry, fatty acid/cholesterol deposition and gene expression (PPARα, LXRα, APOA1, and APOB mRNA levels) were evaluated. Results. The MP+HCC group showed a transiently higher APOA1/APOB ratio in serum, but the hepatic gene expression of both proteins was similar among groups; while MF caused a lower serum level of IL-1α/β, IFN-γ, TNF-α, FDM increased IL-4, IL-6 and IL-10 levels. NASH was more pronounced in HCC (alone) group, but all mango-supplemented diets reduced NASH to a different extent (MP>MF>FDM), a fact associated with a lower cholesterol deposition (-33%) and a different fatty acid profile. A higher PPARα and LXRα mRNA level was found in MP and MF-fed groups, respectively. Conclusions: MP and MF exert synergistic hepatoprotective effects in HCC-fed rats; such effects are more evident when supplemented alone than combined (FDM).




Funding Source:

Mexican Council of Science and Technology (CONACyT), projects 563 (Un enfoque multidisciplinario de la farmacocinética de polifenoles de
mango Ataulfo: Interacciones ,moleculares, estudios preclínicos y clínicos" y CB-2015-1/254063 (Antioxidantes de frutas y verduras en cáncer, ¿Son su bioaccesibilidad, biodisponibilidad o sinergismo estructural responsables de su ineficacia?*.

CoAuthors: J. Abraham Domínguez-Ávila , DSc – Centro de Investigación en Alimentación y Desarrollo A. C; Jacqueline Ruiz-Canizales, MSc – Centro de Investigacion enAlimentacion y Desarrollo, A.C.; Mónica Villegas-Ochoa , Q.B. – Centro de Investigacion en Alimentacion y Desarrollo, A.C.; Norma Salazar-López , DSc – Universidad de Sonora; Gustavo González-Aguilar, PhD – Centro de Investigacion en Alimentacion y Desarrollo, A.C.

Abraham Wall-Medrano

Research-Professor
Universidad Autonoma de Ciudad Juarez - Instituto de Ciencias Biomedicas
Ciudad Juarez, Chihuahua, Mexico