Poster Topical Area: Energy and Macronutrient Metabolism

Location: Hall D

Poster Board Number: 532

P10-133 - Antioxidant profile, first-pass metabolism and anti-proliferative capacity of fruits with graded levels of phenolic compounds

Monday, Jun 11
8:00 AM – 3:00 PM

Objective. To evaluate the antioxidant profile, first-pass metabolism and anti-proliferative capacity of three fruits with graded levels of phenolic compounds. Methods. Phenolic compounds (PC) and antioxidant capacity of globe grape, raspberry and blackberry was evaluated by spectrophotometry and HPLC-ESI-q-TOF-MS. In vitro bioaccesibility of PC subclasses was followed under simulated GI conditions (oral, gastric, intestinal). Real-time ex vivo (gut everted sac) monitoring of first-pass phenolic metabolites and PC's apparent permeability (Papp) were followed by differential pulse voltammetry (0 to 120 min), spectrophotometry and HPLC-ESI-q-TOF-MS (120 min). The anti-proliferative capacity of main PC and metabolites was assayed (MTT). Results. PC richness/fingerprint (cyanidin/pelargodin derivates, catechin, epicatechin), antioxidant (DPPH, FRAP) and electrochemical reactivity and bioaccesibility (total PC and flavonoids) was red blackberry> raspberry> globe grape. In vitro anthocyanins' stability was pH-dependent. Papp of PC from globe grape favored their absorption while PC efflux was favored in raspberry and blackberry. PC's absorption and ex vivo biotransformation was fruit-dependent and quinic acid (raspberry/blackberry), malvidin-3-O-glucoside and caffeic acid (blackberry) were major metabolites but they did not showed antiproliferative capacity in normal retinal, breast, lung and colorectal carcinoma cells. Conclusions. PC's bioaccesibility, chemical stability, first-pass metabolism was fruit richness-dependent but the anti-cancer potential of its metabolites (bioequivalence) is not.



Funding Source: Mexican Council of Science and Technology (CONACyT; project CB-2015-1/254063)

CoAuthors: Francisco Olivas-Aguirre , MSc – Universidad Autonoma de Ciudad Juarez; Sandra Mendoza-Díaz , PhD – Facultad de Quimica, Universidad Autonoma de Queretaro; Arely Cárdenas-Robles, PhD – Facultad de Química, Universidad Autónoma de Querétaro; Emilio Álvarez-Parrilla , PhD – Universidad Autonoma de Ciudad Juarez; Ramón Robles-Zepeda , PhD – Universidad de Sonora; Gustavo Gonzalez-Aguilar, PhD – Centro de Investigacion en Alimentacion y Desarrollo, A.C.; Nina Martinez-Ruiz, DSc – Universidad Autónoma de Ciudad Juarez

Abraham Wall-Medrano

Research-Professor
Universidad Autonoma de Ciudad Juarez - Instituto de Ciencias Biomedicas
Ciudad Juarez, Chihuahua, Mexico