Poster Topical Area: Aging and Chronic Disease
Poster Board Number: 126
Patients with short bowel syndrome (SBS) need total parenteral nutrition (TPN) to maintain their life; however, TPN-associated gut atrophy, bacterial overgrowth and immune dysfunction may cause adverse response. Studies showed that specific components of dead probiotics (paraprobiotics) may have benefits as live probiotics in promoting intestinal health. Short-chain fatty acids (SCFAs), the main fuel of enterocytes, are produced in the fermentation process of live, not dead probiotics. The aim of this study is to investigate the effects of paraprobiotics, SCFAs and extracellular polysaccharidepeptides of Trametes versicolor (TV LH-1 ePSP), the anti-inflammatory and immuno-modulatory agent, on jejunal damage in TPN-supported SBS rats. Male SD rats, except for the normal controls, were performed with intestinal resection from 10 cm distal of the duodenal treitz ligament to 5 cm distal colon and the right jugular vein intubation. TPN was administered right after the surgeries; and oral distilled water, live Lactobacillus acidphilus and Bifidobacterium bifidum, heat-treated dead probiotics (paraprobiotics), or the combination of paraprobiotics and intravenous SCFAs (acetate, propionate and butyrate) with or without oral TV LH-1 ePSP were administered for 6 days to SBS rats. The results showed that SBS rats had significantly decreased body weights, serum glucose, albumin, creatinine and GPT, and circulating red blood cells, hemoglobin and hematocrit compared to the controls (one-way ANOVA, p <.05).Oral consumption of live probiotics and paraprobiotics had no significant impact on serological parameters, circulating blood counts, or jejunal damage. However, SBS-increased serum triglycerides were significantly decreased and SBS-increased relative weights of lung, liver and kidneys were further increased by combined paraprobiotics and SCFAs with or without TV LH-1 ePSP. SBS rats had significantly increased villus height and injury score in the jejunum; however, paraprobiotics, SCFAs and TV LH-1 ePSP had no significant impact on jejunal mass and histology. In summary, combination of paraprobiotics, SCFAs and TV LH-1 ePSP may improve hypertriglyceridemia in SBS rats; however, the SBS-induced intestinal damage were not improved.
Support or Funding Information
Nutritional Science, Fu Jen Catholic University, New Taipei City, Taiwan
New Taipei City, New Taipei, Taiwan (Republic of China)