Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 325
Trauma hemorrhagic shock and reperfusion (THR) is an emergent condition accompanied with a cascade production of reactive oxygen species (ROS) and inflammatory mediators. Glutamine, a precursor of de novo antioxidant glutathione, has been shown to restore hepatic ATP depletion and reduce hepatic damage in THR rats. In addition, n-3 polyunsaturated fatty acids derived from fish oil are the precursors of pro-resolving mediators in inflammation resolution. Therefore, the aim of this study is to investigate the effects of glutamine- and fish oil-supplemented resuscitation fluids on oxidative stress and inflammatory mediators of the liver and lung in THR. THR rats were suffered from 5 cm midline laparotomy and catheterizations in the left carotid artery and right jugular vein; blood drawn from the left carotid artery to reach a mean arterial pressure 30 to 35 mmHg within 10 min and maintained hypovolemia for 60 minutes; and subsequently resuscitated with shed blood and equal volume of lactate Ringer's solution with or without L-alanyl-L-glutamine and/or fish oil within 10 minutes and followed by continuous, low infusion rate of resuscitation fluids. Healthy and sham-operated rats were included as controls. After 42 h of infusion, THR rats had significantly increased relative lung weights and decreased liver glutathione peroxidase (GPx) activity (one-way ANOVA, p<0.05) compared to the controls. The THR-increased hepatic TBARS, i.e., the index of lipid peroxidation, were decreased by glutamine and fish oil and the increased pulmonary TBARS were decreased by glutamine (two-way ANOVA, p<0.05). In THR rats, glutamine was the main factor to increase hepatic interferon-gamma and interleukin (IL)-6 and pulmonary intercellular adhesion molecule (ICAM)-1, non-protein thiol group and GPX activity. Hepatic IL-6 and ICAM-1 and pulmonary IL-4 and ICAM-1 were significantly decreased by fish oil and these effects were attenuated when combined with glutamine. Moreover, combination treatment of glutamine and fish oil significantly decreased pulmonary IL-8. In conclusion, these results suggest that both glutamine- and fish oil-supplemented resuscitation fluids may decrease anti-oxidative capacity and fish oil-supplemented resuscitation fluids may further decrease inflammatory mediators in the liver in THR.
Support or Funding Information
Bing Xiang Liu
Nutritional Science, Fu Jen Catholic University, New Taipei City, Taiwan
New Taipei City, New Taipei, Taiwan (Republic of China)