Poster Topical Area: Diet and Cancer
Poster Board Number: 196
Patients with non-small cell lung cancer (NSCLC) often show a single carbon disordered state, and it has been demonstrated that a low folate microenvironment induces stem cell characteristics in non-small cell lung cancer associated with malignant progression. A549 non-small cell lung cancer cell lines were cultured at high/low folate levels for 4-12 days and anchorage-independent oncospheroid formation was performed to observe the self-renewal of cancer cells. The results showed that the number of tumorspheres formed by low/high folate group was significantly higher than the control group, but the tumor spheres formed by high folate was larger than the low folate group. Folate imbalance caused increased expression of PPIF, STMN2, DYNLL1 (closely related to the formation of tumor cells and cytoskeletal related proteins). Moreover, low folate group with the treatment of metformin and rapamycin enhanced the expression of cytoskeletal markers. However, only Rapamycin enhanced the expression of PPIF, STMN2, and DYNLL1, and Metformin tended to inhibit the cell configuration under the condition in high folate group. In summary, folate disordered promotes self-renewal of NSCLC cells and affects the performance of anti-cancer drugs in inhibiting cytoskeletal markers of cancer metastasis.
Fu Jen Catholic University
New Taipei, New Taipei, Taiwan (Republic of China)