Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 267
Objective: To evaluate the effect of 4-hydroxychalcone (4HC) as well as a combination of 4HC and metformin (Met) on glucose and lipid profile on wistar rats with a normal diet and simple water (W) consumption as well as water with a 50% sucrose concentration (Suc) consumption.
Methods: A total of 48 wistar rats were divided into 8 experimental groups (n=6 per group): 1. W+SS (saline solution), 2. W+Met, 3. W+4HC, 4. W+4HC+Met, 5. Suc+SS, 6. Suc+4HC, 7. Suc+Met and 8. Suc+4HC+Met. The doses of treatments used per day in the groups were 200 mg/kg of Met and 80 mg/kg of 4HC. All the groups received the same diet (Lab Diet 5012, USA). The duration of the study was 24 weeks; A blood sample was collected at the end of the study with a night of fasting. Glu, TAG, TC and HDLc were assessed using commercial kits (RANDOX, United Kingdom). Data were analyzed by analysis of variance (ANOVA) followed by post hoc Tuckey test with p < 0.05 set as significant.
Results: There were no differences in Glu, TAG and HDLc between W+SS and Suc+SS, TC was statistically lower in Suc+SS (p < 0.05); In the groups with W there were no differences between treatments in Glu, TAG and HDLc as compared with W+SS. There were not statistically differences in TC between W+SS and W+Met+4HC. The treatments with Met and 4HC separately, both statistically decreased TC (p < 0.05); No differences were observed between groups with Suc in Glu and TAG, nevertheless TC and HDLc were lower in rats with Suc+Met+4HC when compared with Suc+SS (p < 0.05). Lower HDLc was also observed with Suc+4HC as compared with Suc+SS group (p < 0.05).
Conclusion: The consumption of water with 50% concentration did not produce a negative effect on lipid profile and Glu. 4HC decreases TC when W is consumed but when Suc is consumed, 4HC+Met decreases TC but at the expense of to decrease HDLc. Due to these results, is not possible yet to suggest the use of 4HC as a treatment for lipids or glucose alterations.
Francisco Humberto Castro-Sánchez
Universidad Autónoma de Sinaloa
Culiacán, Sinaloa, Mexico