Poster Topical Area: Vitamins and Minerals

Location: Hall D

Poster Board Number: 529

P26-068 - First trimester B6, folate and B12 concentrations and their associations with serum lipid concentrations in pregnancy: Prospective study

Sunday, Jun 10
8:00 AM – 6:00 PM

Objectives: To evaluate associations between first trimester vitamin B6, folate and B12 concentrations and changes in total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and triglyceride (TG) concentrations throughout pregnancy.


Methods:
Blood samples from 178 pregnant women (20-40y), attending a public health care center in Rio de Janeiro/Brazil were collected during follow-ups at 5-13 (baseline), 20-26, and 30-36wk gestation. Plasma was analyzed for vitamin B6 (nmol/L) by high performance liquid chromatography-fluorescence detection, folate (nmol/L) and B12 (pmol/L) both via commercial Roche e411 bioanalyzer. Serum lipids were measured enzymatically, using commercial kits; LDL-c was calculated using the Friedewald formula. Adjusted longitudinal linear mixed-effects models were employed. Values of vitamins were fixed in the first trimester for the analyzes. Analyzes were adjusted for gestational weeks, age, education, smoking, alcohol, pre-pregnancy energy intake and early pregnancy body mass index.


Results:
Prevalence of B6, folate and B12 adequacy were 94.8%, 99.4% and 81.5%. Mean concentrations (SD) of the in the first trimester were B6: 40.0nmol/L (18.2), folate: 27.0nmol/L (10.2), and B12: 320.6pmol/L (120.8). The mean concentrations (SD) of serum lipids in each trimester (1st, 2nd, 3rd) were TC: 160.6mg/dL (28.9), 210.6mg/dL(35.3), and 224.1mg/dL(41.4); LDL: 96.8mg/dL(21.6), 127.1mg/dL(28.7), and 137.6mg/dL(33.7); HDL: 47.8mg/dL(8.5), 57.1mg/dL(9.9), and 54.9mg/dL(10.0); TG: 79.9mg/dL(32.5), 130.3mg/dL(48.8), and 158.3mg/dL(48.9). In adjusted analyses, folate concentrations were associated with an increase in CT, LDL and TG throughout gestation (β 0.422, 95%CI 0.107-0.737, p=0.009; β 0.282, 95%CI 0.037-0.527, p=0.024; β 0.479, 95%CI 0.093-0.866, p=0.015; respectively), and B12 concentrations were associated with an increase in HDL (β 0.009, 95%CI 0.001-0.017, p=0.035).


Conclusions:
Folate was associated with the increase of CT, LDL and TG during pregnancy, whereas B12 was associated with increase of HDL. No relationships were found between B6 and the lipids. There is a need for research to understand the associations of folate and B12 status and serum lipids changes in pregnancy.




Funding Source: Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro - FAPERJ, National Council for Scientific and Technological Development - CNPq, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES

CoAuthors: Manoela da Silva – Postgraduation Program in Nutrition and Food, Faculty of Nutrition, Pelotas Federal University; Amanda Figueiredo – Nutritional Epidemiology Observatory, Rio de Janeiro Federal University; Luna Vieira – Postgraduation Program in Epidemiology, Faculty of Medicine, Pelotas Federal University; Daniela Hampel – USDA-ARS Western Human Nutrition Research Center, Department of Nutrition, University of California, Davis, CA, USA; Lindsay Allen – USDA-ARS Western Human Nutrition Research Center, Department of Nutrition, University of California, Davis, CA, USA; Gilberto Kac – Nutritional Epidemiology Observatory, Rio de Janeiro Federal University

Juliana S. Vaz

Adjunct Professor
Postgraduation Program in Nutrition and Food, Faculty of Nutrition, Federal University of Pelotas
Pelotas, Rio Grande do Sul, Brazil