Poster Topical Area: Dietary Bioactive Components

Location: Hall D

Poster Board Number: 288

P08-030 - Effects of Mixed Nut Supplementation on Inflammation, Oxidative Stress, Antioxidant Enzymes and Liver Function Markers in Rats Fed an Atherogenic Diet

Monday, Jun 11
8:00 AM – 3:00 PM

Objectives: Cardiovascular disease (CVD) is the number one cause of death in the United States. Inflammation and oxidative stress are key processes in the development of CVD. Nuts are rich in unsaturated fatty acids, plant protein, fiber, vitamins, minerals, and many phytochemicals. These bioactive materials and nutrients improve antioxidant capacity and have anti-inflammatory abilities, which may be associated with the beneficial effects of nuts on CVD risk factors. Several studies have examined the effects of individual nuts on inflammation but there is limited research on whether the beneficial effects would be extended with the consumption of a mixture of nuts, which would provide a greater nutrient variety. Therefore, the objective of this study was to examine the effects of mixed nut consumption on inflammation, oxidative stress, antioxidant capacity and liver function enzymes in rats fed atherogenic (high fat, cholesterol and sugar) diet.


Methods:
Male Sprague-Dawley rats at 28 days of age (total N = 30) were allocated into three groups and fed isocalroic control diet (no nuts), 8.1% pistachio diet (single nut), or 7.5% mixed nut diet (almonds, cashews, pecans, pistachios, walnuts, peanuts, macadamia nuts, and brazil nuts) for 8 weeks.


Results:
Both pistachio and mixed nut groups significantly decreased C-reactive protein as an inflammation marker (P=0.045) and thiobarbituric acid reactive substances (TBARS) as an oxidative stress indicator (P=0.004), and increased total antioxidant capacity (P=0.048) compared to the control group. Mixed nut groups had greater superoxide dismutase (P =0.004) and catalase (P =0.044) and lower aspartate aminotransferase (P =0.048) activities than the control group. There were no significant differences on alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase and creatinine kinase among groups.


Conclusions:
This study suggests mixed nut consumption provides comparable health benefits of those found for individual nut intake with regard to risk factors for CVD, including inflammation, oxidative stress, total antioxidant capacity, antioxidant enzyme activities and liver function markers in atherogenic-diet fed rats.




Funding Source:

American Heart Association (16GRNT31360007)

CoAuthors: Amanda Marx – San Diego State University; Caitlin Rasmussen – San Diego State University

Mee Young Hong

Professor
San Diego State University
San Diego, California