Poster Topical Area: Dietary Bioactive Components

Location: Hall D

Poster Board Number: 366

P08-108 - Administration of Purified Cyanidin-3-Glucose or a Blackberry Extract Causes Improved Mitochondrial Function but Reduced Content in 3T3-L1 Adipocytes

Monday, Jun 11
8:00 AM – 3:00 PM

Objectives: Consumption of berries elicits anti-obesity and anti-diabetic effects in both animal and human studies. The application of anthocyanin or berry extracts to adipocytes in vitro leads to increases in insulin sensitivity, reductions in lipid droplet formation, and a white-to-brown (beige) phenotypic switch of the tissue. The objective of this study was to investigate the effect of cyanidin-3-glucoside (C3G) or blackberry extract (BBE) on respiration in mature adipocytes.

Methods: In two separate experiments, 3T3-L1 adipocyte cells were treated with 0, 150 nM, or 50 µM purified C3G or equivalents provided by a BBE. The BBE experiment also included a 25 µM dose. Baseline, state 4, state 3, and uncoupled respiration states were created experimentally and measured by oxygraph. Respiration rate within each experimental state was corrected for mitochondrial content, which was determined by PCR.

Results: Mitochondrial content was significantly reduced with the high dose of C3G (12.9 vs. 14.1 vs. 7.6 2*2^ΔCt, control vs. 150 nM vs. 25 µM C3G, respectively; p=0.007) as well as with the two higher doses of BBE (14.5 vs. 14.3 vs. 5.5 vs. 4.6 2*2^ΔCt, control vs. 150 nM vs. 25 µM vs. 50 µM, respectively; p=0.0001). After correction for mitochondrial content, per million cells, the 50 µM dose of C3G resulted in the highest respiration rates (control vs. 150 nM vs. 50 mM, respectively) for baseline state (7.3 vs. 5.1 vs. 10.0 pmol O2/s; p=0.0002), state 4 (5.4 vs. 4.0 vs. 7.1 pmol O2/s, p=0.0021), state 3 (38.2 vs. 27.7 vs. 56.1 pmol O2/s; p=0.0013), and uncoupled state (79.1 vs. 57.8 vs. 109.4 pmol O2/s; p=0.0037). The intermediate-high dose (25 mM) BBE also increased respiration (control vs. 150 nM vs. 25 mM vs. 50 mM) for baseline state (5.7 vs. 6.0 vs. 14.2 vs. 7.8 pmol O2/s, p2/s, p=0.0002), state 3 (29.0 vs. 32.0 vs. 60.5 vs. 31.0 pmol O2/s, p=0.0025), and uncoupled state (58.6 vs. 62.0 vs. 89.4 vs. 48.2 pmol O2/s, p=0.0107).

Conclusion: Administration of a purified anthocyanin or a berry extract caused decreases in mitochondrial content, but increases in mitochondrial function in cultured adipocytes. These observations support the anti-obesity effects associated with anthocyanins noted in earlier studies.




Funding Source: Supported by the U.S. Department of Agriculture.

CoAuthors: George Albaugh, PhD – United States Department of Agriculture - Beltsville Human Nutrition Research Center; Dawn Harrison, MS – United States Department of Agriculture - Beltsville Human Nutrition Research Center; Dave Luthria, PhD – United States Department of Agriculture - Beltsville Human Nutrition Research Center; David Baer, PhD – United States Department of Agriculture - Beltsville Human Nutrition Research Center; Janet Novotny, PhD – United States Department of Agriculture - Beltsville Human Nutrition Research Center

Patrick Solverson

Postdoctoral Research Associate
United States Department of Agriculture - Beltsville Human Nutrition Research Center
Beltsville, Maryland