Poster Topical Area: Vitamins and Minerals

Location: Hall D

Poster Board Number: 469

P26-007 - Correlation Between Chylomicron Concentration and Fat Soluble Vitamin Uptake in Metabolic Syndrome and Healthy Subjects

Sunday, Jun 10
8:00 AM – 6:00 PM

Objective: Subjects with metabolic syndrome (MetS) have been shown to absorb less vitamin E than healthy matched controls. Therefore, we hypothesize that the other fat-soluble vitamins (FSVs; vitamins A, D, and K) will follow a similar response.

Chylomicron fractions from a study investigating postprandial absorption of vitamin E after consumption with a soymilk beverage were re-analyzed at hours 0, 3, 6, 9, 12 for FSVs (retinyl palmitate, ergocalciferol, and phylloquinone) and apolipoprotein B-48 (apoB-48) using high-performace liquid chromatography-tandem mass spectrometry (LC-MS/MS) and ELISA, respectively.

Preliminary results from a subset of subjects indicate no significant differences in FSV absorption between MetS and healthy subjects (p = 0.39-0.78 for vitamins A, D, and K). In contrast, there appears to be a correlation between chylomicron vitamin A and D concentration and apoB-48 in MetS subjects but not in healthy subjects (vitamin A: MetS R2=0.45, p=0.02; healthy R2=0.07, p=0.72; vitamin D: MetS R2=0.43, p=0.03; healthy R2=0.14, p=0.45).

These findings suggest that chylomicron enrichment of vitamin A and D concentrations are dependent on apoB-48 in MetS subjects, but not in healthy subjects.To date, there appear to be no differences due to health status in postprandial concentrations of vitamins A or D absorbed. Lipidomic analyses will be performed to understand systematic differences in intestinal absorption of fat-soluble compounds between MetS and control subjects.

Funding Source:

This project was partially supported by a Foods for Health Discovery Themes Seed Grant.

CoAuthors: Priyankar Dey, PhD – The Ohio State University; Richard Bruno, PhD – The Ohio State University; Rachel Kopec, PhD – The Ohio State University

Haley Chatelaine

PhD Student
The Ohio State University
Columbus, Ohio