Poster Topical Area: Energy and Macronutrient Metabolism
Location: Hall D
Poster Board Number: 458
Objectives: Dietary fats are mostly absorbed in the upper gastrointestinal tract, but consumption alters hindgut microbiome composition. The objective of this study was to determine the impact of a combination of saturated medium chain (sMCT) and polyunsaturated long chain triglycerides (pLCT) on serum levels of gut microbial metabolic products (postbiotics) and inflammatory cytokines in cats. Postbiotics can influence inflammatory status, with implications for health and disease.
Methods: Randomized 2X2 design, ± sMCT ± pLCT, 4 groups, n = 16 per group. Cats were fed for 28 days and group housed in environments promoting social interaction, having access to natural light that varied with season and opportunity to exercise. Health was confirmed through clinical indices. Collections were under IACUC approved protocols. Cats were fed to maintain healthy body weight. Serum gut microbiome postbiotics were assessed by metabolomics LC-MS. Inflammatory cytokines were measured by ELISA from whole blood samples cultured in vitro ± lipopolysaccharide (LPS) as a proxy for metabolic endotoxemia.
Results: Multivariate tests by postbiotic class showed sMCT + pLCT, but not either alone, decreased serum indole and phenol postbiotics, as well as sulfated host co-metabolites. Univariate tests exposed drivers; e.g. the putrefaction products 4-ethylphenol sulfate, 4-vinylphenol sulfate, indoleacetate and indolelactate were all significantly decreased by the combination of fats. The combination of sMCT + pLCT also decreased cytokines in whole blood cultures from fed cats: FAS, GM-CSF, IL-2, IL-13, IL-8 and SDF-1 were decreased 15-64% relative to the additive effects of both fats separately in cultures without added LPS. In cultures with added LPS, the cytokines FAS, GM-CSF, IL-2, IL-13, SDF-1 and TNF-alpha were decreased 10-90% relative to the additive effects of both fats separately.
Conclusions: The combination of sMCT and pLCT decreases circulating levels of feline gut microbial postbiotics some of which are uremic toxins, and also decreases levels of inflammatory cytokines in whole blood cultures from fed cats, regardless of culture stimulation by LPS. Dietary sMCT and pLCT fats in combination may improve functional dysbiosis and decrease inflammation in cats over 4 weeks.
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