Poster Topical Area: Obesity
Location: Hall D
Poster Board Number: 706
Objectives: Non-shivering thermogenesis is the main function of brown adipose tissue which burns body fat by generating heat. Thus, activating non-shivering thermogenesis might be a promising way to prevent obesity. Moreover, brown adipogenesis was inversely correlated with a canonical Wnt signaling pathway that is related to the regulation of cell development. The purpose of this study is to determine the effect of quercetin on non-shivering thermogenesis and brown adipogenesis in brown adipocytes through a Wnt signaling pathway.
Methods: Immortalized brown preadipocytes were seeded and then differentiated for 11 days, which becomes mature brown adipocytes (a control). In an experimental group, quercetin (40 µg/ml) was treated during the differentiation (day 5, 7, and 9). Fully matured brown adipocytes (day 11) were harvested for extracting total RNA. Sequentially cDNA was synthesized and then the expression of genes, such as uncoupling protein 1 (UCP1), cell death-inducing DFFA-like effector A (CIDEA), and peroxisome proliferator-activated receptor (PPAR) γ-coactivator 1-α (PGC1α) that are related to non-shivering thermogenesis, carnitine palmitoyltransferase 1α (CPT1α), a main regulator for fatty acid oxidation, and Wnt10b, Frizzled 2, Low-density lipoprotein receptor-related protein (LRP)-6, and β-catenin that are involved in the regulation of a canonical Wnt signaling was measured using a real-time PCR.
Results: Brown adipocytes treated with quercetin showed increased expression of UCP1, CIDEA, and CPT1α genes whereas Wnt10b, Frizzled 2, LRP-6, and β-catenin genes were downregulated.
Conclusions: Quercetin may induce function of brown adipocytes by possibly inhibiting a canonical Wnt signaling pathway.
North Carolina Agricultural and Technical State University
Greensboro, North Carolina