Poster Topical Area: Energy and Macronutrient Metabolism

Location: Hall D

Poster Board Number: 478

P10-078 - Effects of feeding waste water algae on mouse metabolome

Monday, Jun 11
8:00 AM – 3:00 PM

Wastewater from animal and food production commonly contains significant amount of nutrients. Without proper processing, nutrient-rich wastewater is an environmental hazard. Cultivating algae in wastewater reduces pollution and also provides an economic source of feed ingredients. However, the metabolic events occurred after feeding wastewater algae to animals have not been examined in details. In this study, 5 groups of young male mice (n= 8/group) were fed the chows containing 0, 5%, and 10% green algae (Chlorella Vulgaris) grown in wastewater from slaughterhouse and dairy processing facility, respectively, for 28 days. Both sources of algae have elevated protein contents that are rich in glycine and alanine. The metabolic effects of wastewater algae were investigated by growth performance, blood chemistry, and liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. The results showed that daily weight gain, food intake, and blood chemistry, including glucose, triacylglycerol, cholesterol, and blood urea nitrogen, were not affected by algae feeding. Metabolomic analysis of urine, serum, and feces samples identified both dose-dependent and source-specific metabolic changes. In urine, the levels of B vitamins were significantly increased by both algae. In serum, glycine and alanine, together with two essential amino acids, phenylalanine and glycine, were increased by both algae. Short-chain fatty acids in feces were only increased by 10% slaughterhouse algae. Muricholic acid and deoxycholic acid in feces were decreased by both 10% slaughterhouse and 10% dairy algae, while lithocholic acid was decreased only by 10% slaughterhouse algae. Overall, feeding wastewater algae did not elicit apparent negative effect on young mice. The observation of algae-elicited changes in amino acid and microbial metabolism could serve as a foundation for further mechanistic investigations on the biological effects of wastewater algae feeding.

CoAuthors: Chi Chen – University of Minnesota; Roger Ruan – University of Minnesota; Wenguang Zhou – University of Minnesota; Paul Chen – University of Minnesota; Pedro Urriola – University of Minnesota; Gerald Shurson – University of Minnesota; Qingqing Mao – University of Minnesota

Yiwei Ma

University of Minnesota, Twin Cities
St Paul, Minnesota