Poster Topical Area: Diet and Cancer
Poster Board Number: 203
Triple negative breast cancer (TNBC), an aggressive breast cancer phenotype with limited treatment options, is diagnosed in approximately 35,000 new breast cancer cases each year in the United States. Since cancer progression is linked to the supportive role of healthy cells found in the tumor microenvironment (TME), new cancer therapies are being sought that disrupt the recruitment and supportive activity of localized cells such as tumor associated macrophages (TAMs). Sulforaphane (SFN), a well-tolerated isothiocyanate derived from cruciferous vegetables, may prevent the progression of TNBC and alter cell-to-cell communication in the TME. SFN has been shown to directly decrease cell viability in TNBC cell lines and reduce tumor growth in animal models. However, there is a critical need to determine SFN action in the TME of TNBC to assess SFN as a potential component of TNBC treatment and chemoprevention. Objective: The objective of this study was to establish the effect of SFN treatment on cell proliferation and invasive potential of human TNBC cells (MDA-MB-231) grown under the influence of TAMs.
Methods: A cell culture study, employing a conditioned media approach to model non-contact interactions found in the TME, using THP-1 human monocytes and MDA-MB-231 human TNBC cells. THP-1 cells were differentiated in 50% (v/v) cancer-conditioned media to create TAMs. MDA-MB-231 cells were then grown in 50% (v/v) TAM-conditioned media for the remaining experiments. TAM-educated MDA-MB-231 were treated with SFN (10 μM) or vehicle control. Cell proliferation was assessed with an MTT-based colorimetric assay. Invasion biomarkers were assessed via qPCR. Statistical analysis was performed by ANOVA and independent t-tests, and significance was noted at p
Results: Significant reduction in cell proliferation was observed in TAM-educated MDA-MB-231 cells after 24 hours (p=0.0132) and 48 hours (p=0.0190) of SFN treatment, compared to vehicle control.
Conclusions: SFN remained effective at reducing cell proliferation of TNBC cells despite the influence of TAMs. This project provides critical information for future studies involving SFN and the treatment of TNBC.
California State University, Chico