Poster Topical Area: Dietary Bioactive Components

Location: Hall D

Poster Board Number: 304

P08-046 - Fast Absorption of Amino Acids From Whey Protein Hydrolysate VS Whey Protein Concentrate

Monday, Jun 11
8:00 AM – 3:00 PM


Whey is a popular source of supplemental protein due to completeness and quantity of branched chain amino acids (BCAAs). Protein digests into amino acids (AA) at different rates, affecting delivery to, and actions within, peripheral tissues. Currently, few studies have compared plasma appearance of amino acids from whey protein hydrolysate (WPH) to, intact whey protein concentrate (WPC). Therefore, purpose of the investigation was to determine plasma AA appearance between WPH, WPC, and carbohydrate (CHO) control supplements in healthy males with the hypothesis that WPH will elicit greater elevations in circulating AAs than WPC.


In a double-blind crossover design, 15 recreationally active males consumed 0.3g of macronutrient/kg lean soft tissue as WPH (Lacprodan Hydro.365â, degree of hydrolysis, DH:26 Arla Foods), WPC (Lacprodan WPC80, Arla Foods), or maltodextrin (CHO). Blood was collected at pre, 0, 10, 20, 30, 45, 60, 90, 120, and 180 minutes following ingestion. Plasma was extracted and frozen at -80oC until analysis. Samples were derivatized (Phenomenex, Torrance, CA) and analyzed using GC-MS. GC-MS chromatograms were analyzed for AA presence and abundance. Data are reported as mean ± SD.


Significantly (p<0.01) greater plasma appearance of all BCAAs was observed for WPH (+2023.2 au) and WPC (+1558.2 au) than CHO (-580.5 au) with no difference between proteins over 180 minutes. During first 20 minutes, plasma appearance of isoleucine was greater (p<0.001) in WPH (+86.1 au) and WPC (+53.8 au) treatments than CHO (-2.5 au). During first 20 minutes, leucine plasma appearance from WPH (+86.1 au) was significantly greater than WPC (+60.1 au) and both had significantly greater appearance than CHO (+1.2 au). Valine plasma appearance from WPH (+123.4 au) was significantly (p<0.05) greater than WPC (-54.6 au) after 30 minutes and both were significantly different from CHO (+20.7 au). During first 20 minutes, total BCAA plasma appearance from WPH (+334.2 au) was significantly greater than WPC (+59.2 au) and CHO (+19.5 au)


Amino acid plasma appearance was significantly increased after protein consumption when supplementing WPH compared to WPC. Recreationally active males may benefit from consumption of WPH to stimulate muscle repair and maintenance more than WPC.

Funding Source:


Authors gratefully acknowledge financial contributions and product from Arla Foods Ingredients Group P/S, Viby, Denmark.

CoAuthors: Jordan Joy – Texas Woman's University; Nancy DiMarco – Texas Woman's University; Shane Broughton – Texas Woman's University; Robert Wildman – Texas Woman's University; Ulla Mikkelsen – Arla Foods; Roxanne Vogel – Texas Woman's University; John Beatty – Texas Woman's University

Nathaniel Y. Kerr

Texas Woman's University
Denton, Texas