Poster Topical Area: Experimental Animal Nutrition

Location: Hall D

Poster Board Number: 610

E09-02 - High oleic palm oil replacing conventional palm oil during pregnancy and lactation induces different effects on adiposity in male mice offspring in adult life

Monday, Jun 11
8:00 AM – 3:00 PM

Objectives: Conventional palm oil (CPO), that currently represents the most used vegetable oil by food industry, when consumed at critical periods of development, may predispose to metabolic changes in adulthood. High oleic palm oil (HOPO), compared to CPO has a lower content of saturated fatty acids (SFA) and a higher proportion of monounsaturated fatty acids (MUFA). However, the effects of HOPO on health are unclear. We evaluated the consumption of normolipidic diets containing two palm oil varieties during perinatal period on the adiposity of the adult offspring.
Methods: C57BL/6 female mice were fed during pregnancy and lactation with diets containing CPO (CPG); HOPO (HOPG) or soybean oil (control - CG). After weaning, male offspring (n= 10) from all groups received control diet until day 100. Food intake and body weight were evaluated throughout the experimental period. The epididymal (EWAT), retroperitoneal (RPWAT) and mesenteric (MWAT) adipose fat pads were removed and weighed. Adiposity was quantified by micro computed tomography (micro-CT). Fatty acids profile of EWAT was analyzed by gas chromatography.
Results: Occasional differences in consumption were observed in the 1st and 2nd week after weaning (CPG> HOPG and CG), with no significant difference in body mass between groups. Regarding adiposity of the adult offspring, the sum of EWAT, RPWAT and MWAT compartments in CPG was significantly higher in relation to CG (+ 60%) and HOPG (+ 34%). In the analysis of abdominal subcutaneous adiposity by computed tomography, CPG showed greater accumulation of this tissue when compared to CG (+ 124%), while HOPG was statistically equal to CG and CPG. The fatty acids profile of EWAT shows that only the CPG showed lower polyunsaturated fatty acids (PUFA) incorporation, as well as a higher desaturation index (16:1/16:0), when compared to control.
Conclusions: While the consumption of CPO promoted deleterious effects on the accumulation of adiposity and caused changes in the fatty acid profile of adipose tissue of the offspring, the consumption of HOPO presented could be a possible alternative lipid source, with similar outcomes to the control. The data demonstrated that the replacement of SFA by MUFA or PUFA in the diet during the perinatal period may favor the occurrence of more favorable metabolic phenotypes in the offspring.




Funding Source:

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

CoAuthors: Raisa dos Santos – Federal University of Rio de Janeiro; Tarsis Marine – Federal University of Rio de Janeiro; Rafael Sales – Federal University of Rio de Janeiro; Gustavo Chicaybam – Federal University of Rio de Janeiro; Sergio Souza – Federal University of Rio de Janeiro; Fatima Sardinha – Federal University of Rio de Janeiro; Maria das Gracas Tavares-do-Carmo – Federal University of Rio de Janeiro

Patricia C. de Velasco

Postdoctoral researcher
Federal University of Rio de Janeiro - UFRJ
Rio De Janeiro, Rio de Janeiro, Brazil