Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 254
OBJECTIVES: When multiple carotenoids are provided at the same time in a nutritional supplement form, there can be competition for uptake in the digestive system. Separating their release over time can reduce this interaction and enhance bioavailability. Our objective was to utilize an in vitro gut simulation model to evaluate the release dynamics of a combination of sequentially released multicarotenoid beadlets. Four beadlets have been designed, composed of naturally derived alpha (AC) and beta carotene(BC), lutein(LU) and zeaxanthin(ZX), lycopene(LY) and astaxanthin(AX), that sequentially release their contents over gut transit time to boost overall bioavailability.
METHODS: Carotenoids from natural sources were prepared and blended into four types of beadlets. Two beadlet formulations release quickly in the stomach and intestine, astaxanthin and a lutein/zeaxanthin combination. Two other beadlet formulations, lycopene and an alpha/beta carotene combination provide controlled release over a period of 4 hr and 6 hr respectively. Dissolution rates of the controlled release formulations were measured by the manufacturer (Omniactive Health Technologies). The release characteristics of a blend of all four beadlets was determined in the TIM gastrointestinal simulation model (Triskelion).
RESULTS: Dissolution testing showed that the original prototypes released carotenoids to solution over a 6-hour period in the order AX, LU, ZX, LY, AC, BC. The controlled release lycopene proceeded from up to 40% release in the first hour to more than 75% release in 4 hours. The alpha/beta carotene achieved release of up to 20% in 2 hours and more than 80% release in 6 hours. Evaluation in the gut simulation model confirmed maximum release and added detail of concentrations over time in the various intestinal compartments (duodenum, jejunum, ileum).
CONCLUSIONS: The results show separation of maximum concentration of the different carotenoids through the compartments of the gut simulation model. Combined with results of a clinical evaluation of a previous version of the beadlet blend, improved bioavailability from a sequentially spaced carotenoid mix is suggested.
Acknowledgements: Omniactive Health Technologies provided the beadlet production and in vitro dissolution testing.
Sr. Principal Research Scientist
Buena Park, California