Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 295
The aim of this work was to evaluate the effect of a combination of xylooligosaccharides (XOS) and a polyphenol blend containing green coffee extract (SLIM™, Plexus Worldwide) on the modulation of the microbiota composition and metabolism as well as the gut wall activity.
A validated in vitro Human Intestinal Microbial Ecosystem (SHIME®) model, coupled with co-cultures of epithelial cells and macrophages, was used to investigate the prebiotic potential and subsequent immune modulation of long-term administration of a blend containing XOS and polyphenols. The experimental setup of the SHIME run included a two-week start-up period, two-week control period and a three-week treatment period.
SLIM was well fermented along the entire colon leading to increased production of butyrate (+7 mmol/L, 58%) in the ascending colon (AC); acetate, propionate and butyrate (approx. +4 mmol/L each) in the transverse colon (TC); propionate (+4.1 mmol/L) in the descending colon (DC). The treatment led also to a mild decrease in ammonium (-7.5%) and increased lactate (55.5%) levels in the AC. The health-related lactic acid bacteria such as Lactobacilli and Bifidobacteria were indeed stimulated (approx. +2.5 Log/mL or 365-fold, and +2.1 Log/ml or 290-fold, respectively) in the lumen. Additionally, Akkermansia muciniphila increased in the lumen of the proximal colon (+2 Log/mL, or 257-fold). SLIM is also shown a protective effect on Caco-2 barrier function in the distal colon (TC and DC), for which a significant increase (+18.5%) in transepithelial electrical resistance was observed, an indicator of reducing the permeability. Finally, upon fermentation, SLIM was found to have pronounced immune activating properties in vitro (e.g. increase of NF-kB transcriptional activity).
SLIM was well fermented along the entire length of the colon and showed potential prebiotic activities, being able to modulate activity and composition of the gut microbiota. This fermentation profile was correlated with a protective effect on Caco-2 barrier function and pronounced immune activating properties in the distal colon.
T. Alan Jiang
VP, Research and Development