Poster Topical Area: Obesity
Location: Hall D
Poster Board Number: 699
Objectives: Short term E4orf1 protein expression (up to 6 wk) in younger mice (8–16 week old) significantly improves glycemic control, suggesting a very promising anti-diabetic role for the protein. Considering that type 2 diabetes is common in advanced age group and needs chronic treatment, this study determined the potential of E4orf1 to continue improvement in glycemic control after a longer intervention in older mice.
Methods: Transgenic mice with inducible and adipose tissue specific expression of E4orf1 (E4) and non-transgenic control (con) mice were maintained on a 60% (kcal) high fat diet for 20 weeks from 9-month of age to induce hyperglycemia. The E4orf1 expression was induced for the 20-week duration. Glycemic control was determined by intra-peritoneal glucose tolerance test (GTT) at week 0 and 11. At week 20, prior to sacrifice mice were fasted for 4 h followed by blood glucose measurement (t=0). Mice were given a bolus of glucose (1.5 g/Kg) and sacrificed 15 min later (t=15). Blood was collected both at t=0 and t=15 to measure serum insulin.
Results: Compared to con mice, E4orf1 expression improved glucose clearance (1.3 fold faster) in E4 mice at week 11 as determined by product of blood glucose and serum insulin. At week 20, in response to glucose load, E4 mice displayed significantly lower peak glucose (p
Conclusions: E4orf1 has the potential to improve glycemic control in older mice, and the improvement persists even after longer term exposure. These results indicate the potential of E4orf1-based approaches as the treatment for type 2 diabetes, which mainly requires chronic treatment of middle-aged and older individuals
Texas Tech University