Poster Topical Area: Nutritional Immunology

Location: Hall D

Poster Board Number: 847

P21-016 - Dietary intake and local cytokine response are associated with skin barrier restoration of experimental wounds

Sunday, Jun 10
8:00 AM – 6:00 PM

Skin wound models effectively assess in vivo immune responsiveness and function at the wound site by sampling inflammatory markers of wound fluid and measuring the skin barrier's time-to-recovery, respectively. However, the influence of nutrient intake and inflammatory biomarkers on wound healing time have not been characterized.

Objectives: We conducted exploratory, secondary analyses using data from a previous study to identify predictors of skin barrier recovery.

In 38 male participants (21 ± 4 y, BMI 26.3 ± 3.9 kg/m2), we examined the role of pre-study dietary intake derived from 3-day food records, and wound and serum biomarkers on time to skin barrier restoration (days). Assessment of pre-study intake included protein, omega-3 fatty acids, vitamins A, C, and D, and zinc. Wounds were created during a 72-h sleep restriction protocol by removing the top layer of ≤8 forearm blisters induced via suction. Wound fluid was serially sampled for 48-h post-blistering to assess local cytokine responses (IL-1b, IL-6, IL-8, TNF-a, MIP-1a and MIP-1b). Serum was sampled daily during sleep restriction to assess cortisol, growth hormone, C-reactive protein (CRP), and circulating cytokine responses.

In linear regression models adjusting for age, BMI, race, ethnicity, energy intake and study group, omega-3 intake was associated with longer healing time ([beta ± SE] per g/d: 0.70 ± 0.33, P=0.04), and protein intake was associated with shorter healing time (per g/d: -0.02 ± 0.01, P=0.01). Incremental area under the curve values (AUCi) of IL-8 (per logged pg/mL: -1.50 ± 0.43, P=0.01) sampled from the wound sites were associated with shorter healing time. None of the serum biomarkers were significantly associated with healing time.

Omega-3 and protein intake may influence wound healing time, which may be predicted by wound cytokines, but not serum biomarkers. Additional cases are needed to confirm these findings. Results may inform future studies assessing nutritional countermeasures to stress-related effects on healing and provide further evidence of mechanisms related to skin barrier recovery.

Funding Source:

U.S. Army Medical Research and Materiel Command. The views expressed herein are those of the authors and do not reflect the official policy of the Army, Department of Defense, or the U.S. Government.

CoAuthors: Adela Hruby, PhD, MPH – U.S. Army Research Institute of Environmental Medicine; Marques Wilson, MS – U.S. Army Research Institute of Environmental Medicine; Scott Montain, PhD – U.S. Army Research Institute of Environmental Medicine

Tracey J. Smith

Nutrition Scientist
U.S. Army Research Institute of Environmental Medicine
Natick, Massachusetts