Poster Topical Area: Nutrient-Gene Interactions
Location: Hall D
Poster Board Number: 458
Objectives: Our study aimed to screen and identify dietary exosomes that inhibit the activation of NLRP3 inflammasome.
Methods: Exosomes from different plants were purified using ultracentrifugation and incubated with bone-marrow derived macrophages. Their effects on NLRP3 inflammasome were evaluated by measuring IL-1b in the medium and caspase-1 autocleavage in the cell lysate, which are two main downstream events of NLRP3 activation. The dietary exosomes with strong inhibitory effects on NLRP3 inflammasome were further characterized and examined their effects on NLRP3 inflammasome-mediated inflammatory responses in animal models.
Results: We identified that exosomes purified from ginger strongly inhibited activation of NLRP3 inflammasome. The ginger exosomes were around 134 nm in diameter. The inhibitory effects of ginger exosomes on NLRP3 inflammasome activation required long incubation (more than 12 hours), suggesting that the cargos inside of the vesicles mediated the inhibitory effects. Most importantly, the administration of ginger exosomes in mice ameliorated NLRP3 inflammasome-mediated inflammatory responses.
Conclusion: our study identified that ginger exosomes inhibited NLRP3 inflammasome activation in vitro and in vivo.
NIH P20GM104320 Nebraska Center Prevention of Obesity Diseases (NPOD) seed grant and project leader grant
University of Nebraska Lincoln