Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 349
Objectives: Obesity is a disease that affects approximately one-third of American adults, and one of its major underlying causes is inflammation. Various food bioactives have anti-inflammatory properties; and we are specifically interested in delta-tocotrienol (dT3), an isoform of Vitamin E, and tart cherries which are rich in anthocyanins (TCA). We have previously reported that dT3 fed to high fat diet-induced obese mice have reduced fat cell size and adipose tissue inflammation. However, the mechanisms behind these were not studied. Moreover, TCA also exerts anti-inflammatory effects in vitro and in vivo. While both dT3 and TCA possess anti-inflammatory properties, their combinational effects are not yet determined. Hence, we are investigating whether dT3 and TCA reduce inflammation in adipocytes, and if the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) pathway mediates these effects.
Methods: Clonal 3T3L1 preadipocytes and adipocytes were pre-treated for 4 hours with dT3 and/or TCA, then stimulated with LPS plus dT3 and/or TCA for another 18 hours. Cells were treated with up to 5 µM of dT3 and up to 16 µg/mL of TCA from TC extracts. These doses were chosen based on previously established dose response and cell viability assays using MTT assays. Total RNA and proteins were isolated for gene and protein analyses. Western blotting was conducted to assess NF-kB activation via P65 phosphorylation.
Results: Doses of dT3 up to 25 µM were not toxic to preadipocytes or adipocytes and none of the tested TCA doses were toxic. In adipocytes, combinations of dT3 and TCA significantly reduced secreted IL-6, more that individual treatments. Lower doses of dT3 and TCA (1 µM of dT3 and 18 µg/mL TCAs) had the most significant reduction in inflammation among all dose combinations tested, when compared to control. Similar results were obtained in preadipocytes. Moreover, using the above doses, TCA but not dT3 significantly reduced LPS-induced P-65 phosphorylation.
Conclusions: dT3 and TCA exerts individual and combinatorial anti-inflammatory effects, which may be beneficial in treating and/or preventing obesity. Additional experiments are ongoing to determine specific pathways mediating these effects of dT3 and TCA, and the mechanistic basis for their interactions.
Research Assistant Professor
Texas Tech University