Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 320
Obejective: Resveratrol has been reported to exert various health-promoting activities, including protective effects against colonic inflammation. Meanwhile it has been known that the oral bioavailability of resveratrol is very low. In order to understand the mechanism underlying this paradox (low bioavailabiltiy but high bioactivity) of resveratrol, we identified and quantified the major metabolites of resveratrol in mouse colon and determined their anti-inflammatory effects in comparison with those of resveratrol.
Methods:Major colonic metabolites were identified and quantified with HPLC-MS. To understand the role of colonic metabolites of resveratrol in anti-inflammation, the inhibitory effects of the metabolites, resveratrol and their combinations at the concentrations equivalent to those found in the mouse colonic tissues were determined in lipopolysaccharide-induced inflammation in both RAW264.7 macrophages and a cell line with TLR-4 specific inflammation.
Results:Our results showed that the major metabolites found in the colonic mucosa of the mice fed physiologically relevant amount of resveratrol were dehydroresveratrol and lunularin. The tissue levels of these metabolites were about 25-fold higher than that of resveratrol in the mouse colonic mucosa. The cell culture results demonstrated that the anti-inflammatory effects of the colonic metabolites were much stronger than that of resveratrol at the relevant tissue levels, moreover, the combination of the colonic metabolites and resveratrol produced the strongest anti-inflammatory effects and the contribution of resveratrol was marginal. It was also found that the anti-inflammatory effects of the metabolites were specific to TLR-4 mediated pathways.
Conclusions:Overall, this study revealed that dehydroresveratrol and lunularin were the major colonic metabolites of resveratrol, and they potentially play much more important roles in anti-inflammation in the colon than resveratrol.
Funding Source: USDA
University of Massachusetts-Amherst