Poster Topical Area: Dietary Bioactive Components

Location: Hall D

Poster Board Number: 284

P08-026 - Dietary Malate or Lactate Attenuates High-Fat Refined Diet Induced Adiposity, Insulin Resistance, and Fatty Liver in Male C57BL/6J Mice

Monday, Jun 11
8:00 AM – 3:00 PM

Objective: Organic acids, such as malate and lactate, may be important contributors to health because they are known to promote energy metabolism. Examples of foods that contain abundant amounts of these compounds include fruit and fermented food products. These dietary "energy acids" may confer some of the health benefits normally associated with these foods (e.g. normalizing insulin sensitivity, weight maintenance, and metabolic health). The objective of this study is to determine, in a diet-induced obesity mouse model, the metabolic effects of two energy acids (malate and lactate) when provided through drinking water.

Methods:
Male C57BL/6J mice (N=36, 9/group) were provided ad libitum for 16 weeks a standard rodent chow or a high-fat refined diet (HFRD, 45.9% kcal fat) with drinking water alone, or a HFRD with drinking water supplemented with either malate or lactate at a food-relevant concentration (100 mM, titrated to pH 7.4 with potassium hydroxide). At the end of 16 weeks, body weight, adiposity, insulin resistance, dyslipidemia, and hepatic lipid content were measured.

Results:
Body weight (P<0.05) and adiposity (visceral, perirenal, epididymal, and subcutaneous, P<0.05) increased with HFRD feeding compared to chow. They were also more insulin resistant (decreased HOMA-IR, P<0.01) and had higher plasma total cholesterol (P<0.01) but not plasma triglycerides (P>0.05), although hepatic triglycerides were higher (P<0.01). Both malate and lactate supplemented mice on the HFRD had blunted weight gain (P<0.05) and adiposity (P<0.05). Moreover, mice supplemented with either energy acid were more insulin sensitive (P<0.05) and had lower plasma total cholesterol (P<0.05) compared to HFRD feeding alone. Finally, the rise in hepatic triglycerides with HFRD feeding was attenuated when mice were supplemented with either malate (P<0.05) or lactate (P<0.05).

Conclusions:
Our data suggests that supplementation with food-relevant levels of either malate or lactate protects mice from high-fat refined diet induced weight gain, insulin resistance, and dyslipidemia. This implies that the metabolic benefits attributed to health-promoting foods like fruit or fermented products may in part be due to their energy acid content and should therefore be considered when assessing a food's nutritional quality.





Funding Source: Bruce and Giovanna Ames Foundation

CoAuthors: Shiva Barforoshi – Children's Hospital Oakland Research Institute; Hellen Ouyang – Children's Hospital Oakland Research Institute; Mark Shigenaga – Children's Hospital Oakland Research Institute

John Gieng

Assistant Professor
San José State University
San José, California