Poster Topical Area: Vitamins and Minerals

Location: Hall D

Poster Board Number: 514

P26-053 - Effects of supplementing chromium-histidinate and picolinate complexes along with biotin on insulin sensitivity in rats fed a high-fat diet

Sunday, Jun 10
8:00 AM – 6:00 PM

Objective: The objective of this work was to investigate the effects of chromium histidinate and chromium picolinate supplementation along with biotin to a high-fat diet (HFD) fed to rats on the insulin sensitivity and the anti-obesity properties.


Methods:
Forty-two Sprague–Dawley rats were divided into six groups (n=7). The rats were fed either 1: a standard diet as control (Control) (12% of calories as fat) or 2: a high-fat diet (HFD) (42% of calories as fat) or 3: a HFD with biotin (300 μg/kg BW per d) (HFD+B) or 4: a combination of HFD and biotin along with CrPic (80 μg CrPic/kg BW per d) (HFD+B+CrPic) or 5: a combination of HFD and biotin along with CrHis (130 μg CrHis/kg BW per d) (HFD+B+CrHis) or 6: a combination of HFD and biotin along with CrHis (65 μg CrHis/kg BW per d) and CrPic (40 μg CrPic/kg BW per d) (HFD+B+CrHis+CrPic).


Results:
Feeding rats a HFD compared with control resulted in an increase in BW, visceral fat, serum glucose, insulin, FFA, leptin, total cholesterol and triglycerides (P = 0.0001). Adding biotin alone or biotin with chromium to HFD decreased (P = 0.0001) the levels of these parameters, with HFD+B+CrHistreatmentbeingthe most effective. Serum, liver and brain tissue Cr concentrations increased upon Cr supplementations (P = 0.0001). Supplementing CrHis along with biotin to a HFD (HFD+B+CrHis) provided the greatest levels of GLUT-1, GLUT-3, PPAR-γ and IRS-1 but the lowest level of NF-κB in the brain tissues, and the greatest levels of GLUT-1, GLUT-3 and PPAR-γ in the liver tissues.


Conclusions:
Biotin supplementation alone or with chromium complexes, CrHis at particular, to a HFD pose to be a potential therapeutic feature for the treatment of insulin resistance and in the prevention of diabetes and its secondary complications.




Funding Source: Nutrition 21

CoAuthors: Cemal Orhan – Firat University; Osman Kucuk – Erciyes University; Mehmet Tuzcu – Firat University; Nurhan Sahin – Firat University; James Komorowski – Nutrition21; Kazim Sahin – Firat University

Nurhan Sahin

Professor
Firat University
Elazig, Elazig, Turkey