Poster Topical Area: Maternal, Perinatal and Pediatric Nutrition
Location: Hall D
Poster Board Number: 376
Objective: To evaluate whether the method of collection of breast milk influences the concentration of macronutrients in the colostrum.
Methods: A prospective, randomized, cross-over study was conducted from March 2017 to January 2018. Colostrum was collected from mothers with excess milk production. The samples were collected up to the seventh day postpartum, in the same puerperal woman, simultaneously by the two methods: in one breast by hand extraction and in the other breast by breast pump, chosen by randomization method. Macronutrients were analyzed using mid-infrared spectroscopy (ref). It is important to note that the milk was collected from the same puerperal woman at the same time and in the same volume in each breast, so the only difference between the samples was the method of collection. Differences were considered statistically significant for p values < 0.05. The calculated n sample (65 samples) was based on a difference in fat concentration of 0.5g/%, alpha error of 10% and beta error of 20%. The results were described with measures of central tendency and the means macronutrient concentrations of the colostrum were compared by the test for paired measurements of Wilcoxon, using the SPSS16.0 software package (SPSS Inc., Chicago, IL). The study protocol was approved by the Ethics Committee and written informed consent has been obtained from the mothers before the collection of the colostrum.
Results: Eighty six samples of human milk collected by breast pump or hand expression were analyzed. There was a difference between lipid concentrations (p = 0.001), significantly higher in milk collected by hand expression (2,4 vs 1,9g/%). There was no statistical difference between the carbohydrate (p = 0.152) and protein (p = 0.937) concentrations between the samples collected.
Conclusion: Protein and carbohydrate concentrations are not affected by the method of collection of breast milk. However, colostrum collected with manual extraction presents higher concentration of lipids comparatively to those collected with electric breast pump.
UNIVERSIDADE FEDERAL FLUMINENSE
Niteroi, Rio de Janeiro, Brazil