Poster Topical Area: Experimental Animal Nutrition

Location: Hall D

Poster Board Number: 614

P11-001 - High-protein exposure during gestation – Consequences on food preferences and health in adult rat offspring with dietary self-selection

Monday, Jun 11
8:00 AM – 3:00 PM

Objectives: Perinatal exposure via mother's diet induces imprinting that impacts longer term phenotype and health of the offspring. This study evaluates the consequences of a maternal high protein diet during gestation on food preference and health markers in rat pups exposed to dietary self-selection (DSS).


Methods:
Two groups of Wistar rat dams were fed a high-protein (HP; 55% protein) or a normal protein (NP; control; 20% protein) isocaloric diet during gestation and then switched to a NP diet during lactation. At 3 wk, female pups from the 2 dam groups were weaned and subdivided into 3 diet groups (i.e. six groups each n=8 pups) until 15 wk. Each group was subjected to either the control NP diet or DSS with a C1 choice diet, or a C2 choice diet, respectively. C1 and C2 groups were exposed to a choice between 2 cups with either proteins (P) or a mix of carbohydrate (C) (88%) and lipid (L) (12%), or a choice between 3 cups, with either protein (P), carbohydrates (C) or lipids (L), respectively.


Results:
Compared to the NP group and independent of maternal diet, C1 and C2 groups consumed 10% more energy as P and decreased C intake (Pfigure 1). C1 groups gained less weight than control NP groups (P+7.7 g; HP-C2, 208.1+11.5 g, P+2.8 g; HP-C2, 62.4+6.8 g, P=0.03) and visceral adipose tissue was increased of 52.1% (NP-C2, 25.9+1.9 g; HP-C2, 39.4+4.5 g, P=0.008) compared to NP-C2 group (figure 2).


Conclusion:
The present study showed model of DSS had a significant impact on possibilities of macronutrients repartition inducing hyperphagia when sources were separated. Adult pups came from HP gestation dam group and under DSS with separated sources had a non adaptative response to food for their homeostatic energy requirments conducting to a higher risk of obesity.



Funding Source:

This work is funded by the ALIAS project, INRA DID'IT metaprogram and Dannore Nutricia Research.


Figure 1 : Total energy intake ingested at 15 weeks. NP, Normal-Protein (control); HP, High Protein; C1 : choice number 1 with 2 cups; C2, choice number 2 with 3 cups; NS, non significant. Data are means + SEMs. a,b Mean values with unlike superscript letters were significantly different between the groups (P < 0.05).

Figure 2 : Visceral adipose tissue at 15 weeks. NP, Normal-Protein (control); HP, High Protein; C1 : choice number 1 with 2 cups; C2, choice number 2 with 3 cups; NS, non significant. Data are means + SEMs. a,b Mean values with unlike superscript letters were significantly different between the groups (P < 0.05).

CoAuthors: Catherine CHAUMONTET, PhD – INRA; Corine DELTEIL – AgroParisTech; Andrea KODDE, MsC – Danone; Daniel TOME, PhD – AgroParisTech; Anne-Marie DAVILA, PhD – AgroParisTech

Gabrielle CARLIN

PhD student
UMR PNCA, AgroParisTech, INRA, Université Paris-Saclay, 75005, Paris, France
PARIS, Ile-de-France, France