Poster Topical Area: Experimental Animal Nutrition
Location: Hall D
Poster Board Number: 614
Objectives: Perinatal exposure via mother's diet induces imprinting that impacts longer term phenotype and health of the offspring. This study evaluates the consequences of a maternal high protein diet during gestation on food preference and health markers in rat pups exposed to dietary self-selection (DSS).
Methods: Two groups of Wistar rat dams were fed a high-protein (HP; 55% protein) or a normal protein (NP; control; 20% protein) isocaloric diet during gestation and then switched to a NP diet during lactation. At 3 wk, female pups from the 2 dam groups were weaned and subdivided into 3 diet groups (i.e. six groups each n=8 pups) until 15 wk. Each group was subjected to either the control NP diet or DSS with a C1 choice diet, or a C2 choice diet, respectively. C1 and C2 groups were exposed to a choice between 2 cups with either proteins (P) or a mix of carbohydrate (C) (88%) and lipid (L) (12%), or a choice between 3 cups, with either protein (P), carbohydrates (C) or lipids (L), respectively.
Results: Compared to the NP group and independent of maternal diet, C1 and C2 groups consumed 10% more energy as P and decreased C intake (Pfigure 1). C1 groups gained less weight than control NP groups (P+7.7 g; HP-C2, 208.1+11.5 g, P+2.8 g; HP-C2, 62.4+6.8 g, P=0.03) and visceral adipose tissue was increased of 52.1% (NP-C2, 25.9+1.9 g; HP-C2, 39.4+4.5 g, P=0.008) compared to NP-C2 group (figure 2).
Conclusion: The present study showed model of DSS had a significant impact on possibilities of macronutrients repartition inducing hyperphagia when sources were separated. Adult pups came from HP gestation dam group and under DSS with separated sources had a non adaptative response to food for their homeostatic energy requirments conducting to a higher risk of obesity.
This work is funded by the ALIAS project, INRA DID'IT metaprogram and Dannore Nutricia Research.
UMR PNCA, AgroParisTech, INRA, Université Paris-Saclay, 75005, Paris, France
PARIS, Ile-de-France, France