Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 372
Effect of Pueraria tuberosa on Experimental Models of Chronic Disease i.e. Diabetic Nephropathy
Yamini B. Tripathi, Rashmi Shukla
Department of medicinal chemistry, Institute of Medical Sciences, Banaras Hindu University, India
Multiple causative factors are involved in mechanism of diabetic nephropathy (DN) the current therapies are not proved perfect medication for DN patients.. Medicinal plants and their bioactive constituents could be considered to be more safe and effective against various chronic diseases like DN. This study was designed to investigate the protective effect of Pueraria tuberosa, a traditional Ayurveda medicine used for antiaging, on diabetic nephropathy in a rat model, and to explore the possible mechanism involved behind its nephroprotective function.
The chemical compositions of aqueous extract of Pueraria tuberosa (PTY-2r) were analyzed by gas chromatography-mass spectrometry (GC-MS). STZ (55mg/kg body Weight, i.p.) induced rats were randomly divided into 3 groups. PTY-2r was orally given to rats for 20 days. Age-matched normal rats served as normal control (NC). Reactive oxygen species (ROS), lipid peroxidation and the activities of ROS-scavenging enzymes – SOD, CAT & GPx were determined in kidney tissue. The expression of apoptosis-related proteins was measured through immunofluorescence.
GC-MS analysis of PTY-2r indicated the presence of thirty-seven compounds among them fourwas found in higher amount. A significant increase in ROS, and LPO was observed along with the decreased activity of antioxidant enzymes which is responsible for oxidative stress in the kidney of DN rats. Since, high oxidative stress induces apoptosis in target cells, as shown by significantly decreased expression of Bcl-2 along with increased expression of Bax, active Caspase-3 & cleaved PARP-1 in DN control rats, suggesting apoptosis. The PTY-2r treatment significantly raised the activity of antioxidant enzymes and suppressed apoptosis thus, prevents urinary albumin excretion in a dose-dependent manner.
These findings suggest that PTY-2r exerts the nephroprotective potential against STZ induced DN rats via suppressing oxidative stress-induced apoptosis due to the presence of different bioactive compounds.
Banaras Hindu University
Varanasi, Uttar Pradesh, India