Poster Topical Area: Diet and Cancer
Poster Board Number: 228
Imbalance of one-carbon donor nutrients in combination with inflammation have been implicated in cancer development. Our previous studies have witnessed effects of folate and choline malnutrition in promoting cancer stem cell signaling network of primary colonic tumors in a colitis-associated mouse model. We hypothesized that imbalanced one-carbon nutrition may induce differential tissue metastasis susceptibility and metabolic reprogramming in a colitis-associated colon cancer mouse model. C57BL/6 mice were fed a control, low folate (LF), low folate and low choline (LFLC) or high folate (HF) diet for 3 months. Mice were treated with dextran sodium sulphate (DSS) and azoxymethane (AOM) to induce colon inflammation and were injected with Lewis lung carcinoma cells to establish cancer metastasis model. Tumor and paired non-tumor tissues of liver and spleen were collected for analysis. The data revealed that the LF group had a higher rate of carrying both liver and spleen metastasis tumor. The LFLC group bear more spleen metastasis tumor. The HF diet tended to have an protective effect against colon tumor development, yet did not exert an protective effect on cancer metastasis. The LFLC diet induced metabolic reprogramming into the Warburg effect in the metastatic microenvironment on hepatic tissue, up-regulating ATP citrate lyase (ACLY) and down-regulating lactate dehydrogenase (LDH) protein expression. Data to address the distinct tissue metastasis susceptibility and metabolic reprogramming induced by imbalanced one-carbon nutrition will be further discussed.
Fu Jen Catholic University
New Taipei City, New Taipei, Taiwan (Republic of China)