Poster Topical Area: Nutrient-Gene Interactions

Location: Hall D

Poster Board Number: 438

P17-015 - Genome-wide analysis of DNA methylation in intervention study of traditional Korean diet

Sunday, Jun 10
8:00 AM – 6:00 PM

Objectives

The objective of the study was to understand the relation between metabolic diseases and epigenetic changes due to diet by investigating the DNA methylation changes in premenopausal women on traditional Korean diet (K-diet).



Methods

We recruited ten overweight perimenopausal women and they were randomly divided into two groups: K-diet (n=5), which is characterized as high consumption of multigrain rice, various fermented foods, vegetables, legumes, fish, and low red meat and control diet group (n=5), consumed Westernized Korean diet. All subjects were stayed at a same metabolic unit for 2 weeks of dietary intervention. Clinical indicators including total blood cholesterol, fibrinogen, insulin, and platelet were measured as instructions in blood collected at baseline, 1wk and 2wks. Whole genome bisulfite sequencing was performed in blood cell DNA. Trimmed reads were aligned and methylation called using BS-seeker2 with Bowtie2. Differentially methylated regions (DMR) between groups were analyzed in 100bp with 50bp step size window and annotated by HOMER. Spearman correlation was analyzed with corrplot R package. Bisulfite amplicon sequencing was used to validate the methylated region.



Results

Two weeks of K-diet significantly reduced blood total cholesterol levels (239.40 ± 15.14 vs 198.20 ± 13.25, p = 0.0163) but not control diet. A significant interaction between time and diet group was observed in the total blood cholesterol (p interaction = 0.0034). A total 85976 DMR (K-diet) and 87621 DMR (control diet) were differentially methylated with significant. (p < 0.05, methylation differences > 5%). We identified 13 DMR where differentially methylated and significant difference were proved with bisulfite amplicon sequencing between K-diet and control diet. Among 13 DMR, spearman correlation test revealed that there were statistical significance in correlation between 8 DMR and clinical indicators and 5 DMR were significantly correlated with clinical dietary compositions (11 food groups, 44 nutrients).

Conclusions

It indicates that DNA methylation in blood cells was significantly regulated by dietary pattern. We suggest that traditional Korean diet can modulate metabolic diseases-associated genes highly correlated with clinical indicators and dietary compositions with modification of DNA methylation.




Funding Source: Korea Food Research Institute

CoAuthors: Jae-jo Park – Korea Food Research Institute; Hee-Sook Park – Korea Food Research Insutite; Soon-Hee Kim – Korea Food Research Institute; Sang-Woon Choi – CHA University; Hae-Jeung Lee – Gachon University; Seon-Joo Park – Gachon University; Phil-Kyung Shin – CHA University; Myung-Sunny Kim – Korea Food Research Institute, University of Science and Technology

Moonju Hong


Korea Food Research Institute in University of Science and technology
Ansan, Kyonggi-do, Republic of Korea