Poster Topical Area: Diet and Cancer

Location: Auditorium

Poster Board Number: 212

P07-026 - Effect of chronic sucrose consumption at human-relevant levels on metabolic health and body composition in mice

Monday, Jun 11
8:00 AM – 3:00 PM

Objectives: To test the hypothesis that chronic consumption of added sucrose at 25% total energy in an ad libitum diet results in impaired metabolic health and adverse body composition of mice compared with a diet with 10% energy from sucrose.


Methods:
We randomized 20 C57BL6 mice into 2 groups from weaning (week 4), where 1 group consumed chow with 10% energy from sucrose (LS) and the other groups consumed chow with 25% energy (HS) from sucrose ad libitum. The two types of chow were isocaloric, and customized based on the AIN-93G/M diet, where sucrose was isocalorically replaced maltodextrin to achieve the desired percentage energy from sucrose. Data collection occurred between weeks 4 to 36. Food and water intakes and body weight of the mice were recorded weekly. Body fat and lean body mass content of the mice was assessed using an NMR-based body composition analyzer every 4-5 weeks. Fasting tail blood glucose was assessed using a glucometer every 3-5 weeks. Difference between groups was assessed using ANCOVA, adjusting for baseline value. A p < 0.05 was set to indicate statistical significance.


Results:
No significant difference was observed in mean ± SEM body weight (HS vs. LS: 43.5 ± 0.9 vs. 44.0 ± 0.9 g), body fat (31.7 ± 0.6 vs. 32.0 ± 0.6%), lean body mass (44.0 ± 0.6 vs. 45.1 ± 0.6%), food intake (33.5 ± 0.6 vs. 33.0 ± 0.5 g/week), and water intake (12.6 ± 1.0 vs. 13.4 ± 1.0 g/week) between groups at week 36 (all p > 0.05). When expressed as accumulated change from week 4, there was also no difference between groups in these variables. There was no difference in total energy intake in the 32-week post-weaning period (14.3 ± 0.2 vs. 14.3 ± 0.2 MJ; p > 0.05). Fasting glucose levels at week 36 were lower in HS compared with LS (6.24 ± 0.3 vs. 8.0 ± 0.3 mmol/L; p < 0.001).


Conclusions:
In an ad libitum setting, chronic consumption of a higher added sucrose diet does not appear to induce metabolic impairment and obesity in mice compared with a lower sugar diet. Our results challenge the common belief that high sugar consumption promotes overeating and is obesogenic, which warrants further research.



Funding Source: Internal funding
Figure 1 - Body weight

CoAuthors: Iris Mei Ying Tse – The University of Hong Kong; Wai Hung Sit, PhD – The University of Hong Kong; Edmund Li, PhD – The University of Hong Kong; Jennifer Man Fan Wan, PhD – The University of Hong Kong; Chi Bun Chan, PhD – The University of Hong Kong

Jimmy Chun Yu Louie

Assistant Professor
The University of Hong Kong
Pokfulam, Not Applicable, Hong Kong