Poster Topical Area: Diet and Cancer
Poster Board Number: 212
Objectives: To test the hypothesis that chronic consumption of added sucrose at 25% total energy in an ad libitum diet results in impaired metabolic health and adverse body composition of mice compared with a diet with 10% energy from sucrose.
Methods: We randomized 20 C57BL6 mice into 2 groups from weaning (week 4), where 1 group consumed chow with 10% energy from sucrose (LS) and the other groups consumed chow with 25% energy (HS) from sucrose ad libitum. The two types of chow were isocaloric, and customized based on the AIN-93G/M diet, where sucrose was isocalorically replaced maltodextrin to achieve the desired percentage energy from sucrose. Data collection occurred between weeks 4 to 36. Food and water intakes and body weight of the mice were recorded weekly. Body fat and lean body mass content of the mice was assessed using an NMR-based body composition analyzer every 4-5 weeks. Fasting tail blood glucose was assessed using a glucometer every 3-5 weeks. Difference between groups was assessed using ANCOVA, adjusting for baseline value. A p < 0.05 was set to indicate statistical significance.
Results: No significant difference was observed in mean ± SEM body weight (HS vs. LS: 43.5 ± 0.9 vs. 44.0 ± 0.9 g), body fat (31.7 ± 0.6 vs. 32.0 ± 0.6%), lean body mass (44.0 ± 0.6 vs. 45.1 ± 0.6%), food intake (33.5 ± 0.6 vs. 33.0 ± 0.5 g/week), and water intake (12.6 ± 1.0 vs. 13.4 ± 1.0 g/week) between groups at week 36 (all p > 0.05). When expressed as accumulated change from week 4, there was also no difference between groups in these variables. There was no difference in total energy intake in the 32-week post-weaning period (14.3 ± 0.2 vs. 14.3 ± 0.2 MJ; p > 0.05). Fasting glucose levels at week 36 were lower in HS compared with LS (6.24 ± 0.3 vs. 8.0 ± 0.3 mmol/L; p < 0.001).
Conclusions: In an ad libitum setting, chronic consumption of a higher added sucrose diet does not appear to induce metabolic impairment and obesity in mice compared with a lower sugar diet. Our results challenge the common belief that high sugar consumption promotes overeating and is obesogenic, which warrants further research.
Jimmy Chun Yu Louie
The University of Hong Kong
Pokfulam, Hong Kong