Poster Topical Area: Experimental Animal Nutrition
Location: Hall D
Poster Board Number: 625
Objectives: The objective of this study was to chemically identify and quantify structural changes in porcine milk oligosaccharides (PMOs) during lactation in pigs in their first and multiple pregnancies.
Methods: 2 to 5 mL of milk sample at 3 time-point of lactation (colostrum at day 1, transitional milk at day 3 and mature milk at day 15 of lactation) was collected from each gilt (n=7) and sow (n=10). The oligosaccharide fractions form the milk was separated using solvent extraction method (chloroform/methanol -2:1) which were then analysed using Isotope-labelling associated LC-ESI-MS/MS.
Results: We reported 55 PMOs structures, of which 25 are new (17 sialylated and 8 neutral). Their incidence in gilt and sow colostrum was almost identical (53 vs. 54), but not in transition milk (48 vs. 53) nor mature milk (41 vs. 47). These PMOs including neutral-, sialyl- and fucosyl- MOs in colostrum were more abundant in the gilt than the sow, but always decreased during lactation. Structural diversity also decreased, although fucosylated MO were conserved.
Conclusion: Our findings infer a key mechanism influencing the observed phenotypes of sow produces significantly more milk and had a more successful pregnancy outcome than gilt in pig reproductions and will assist in the future identification of mechanisms responsible for more successful pregnancy outcomes in women with second babies.
Professor of Physiology and Nutrition
Charels Sturt University
Wagga Wagga, New South Wales, Australia