Poster Topical Area: Vitamins and Minerals
Location: Hall D
Poster Board Number: 521
Objective: The present study aims to assess associations between inflammatory markers as cytokines and adhesion molecules, and circulating folic acid (FA) among population exposed to mandatory fortification of flour with folic acid.
Methods: Data was from a cross-sectional population-based survey conducted in 2008 from 302 adult residents of São Paulo City, Brazil. The country has mandatory fortification of wheat and corn flours with folic acid since 2012 (150 µg per 100g of flour). Circulating folic acid, i.e. unmetabolized folic acid, was assayed by a modified affinity-HPLC method with electrochemical detection to measure the different forms of the vitamins in the plasma. We used the MILLIPLEX MAP 13-plex Cytokine Kit and Human CVD Magnetic Bead Panel 2 from Merck Millipore Corp to analyze the cytokines (IL-1β, IL-6, IL-8, IL-10, IL-12) and adhesion molecules (ICAM and VCAM), respectively. Data analyses were performed using STATA (version 13.0). Multivariate logistic regressions were applied to investigate the association between inflammatory markers (categorized in two categories by the median as cutoff) and circulating FA (tertiles).The model was additionally adjusted for sex, smoking, sex, age and BMI.
Results: The prevalence of detectable circulating FA in this population was high (81.2%), with median concentration of 0.74 ng/ml. Median of circulating FA for 1st, 2nd and 3rd tertiles were 0, 0.72 and 1.45 ng/ml, respectively. The medians of inflammatory markers were: 139.02 ng/ml for ICAM, 869.97 ng/ml for VCAM, 0.38 pg/ml for IL-1β, 2.04 pg/ml for IL-6, 2.47 pg/ml for IL-8, 15.74 pg/ml for IL-10, 3.95 pg/ml for IL-12. The odds ratios (95% CIs) for circulating FA in the highest tertile were: 1.35 (0.73;2.47) for ICAM, 1.19 (0.66;2.14) for VCAM, 0.46 (0.25;0.83) for IL-1β, 0.81 (0.45;1.47) for IL-6, 1.31 (0.73; 2.37) for IL-8, 0.65 (0.36;1.17) for IL-10, 0.51 (0.28;0.92) for IL-12.
Conclusions: After multivariate adjustment, significant association was observed between cytokines (IL-1β and IL-12) and circulating folic acid. Although the vitamin is not metabolized, the presence of circulating FA may indicate an adequate status because this form is only identified with saturation of the folate metabolism due to the excessive intake of this vitamin.
Financial Support from Municipal Health Secretariat of São Paulo; National Counsel of Technological-CNPq; and Scientific Development and São Paulo Research Foundation – FAPESP (Grant Number: #2010/19899-5, #2011/19788-1 #2012/05505-0 and #2015/12196-2).
Department of Nutrition, School of Public Health, University of São Paulo
São Paulo, Sao Paulo, Brazil