Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 292
Objective: Dietary bioactive compounds with antioxidant and anti-inflammatory properties may prevent obesity-associated inflammation and metabolic disorders. Our objective is to understand the mechanisms mediating beneficial effects of tart cherry anthocyanins (TCA) in metabolic disorders. We hypothesized that TCAs reduce obesity-associated inflammation through direct anti-inflammatory effects in adipose tissue.
Methods: Male Zucker fatty rats were fed a diet supplemented with 4% w/w TC powder while the control group fed with normal chow diet for 8 weeks. Serum and adipose tissues were collected to determine anti-inflammatory effects of TC supplementation. Optimal TC concentration and its effects on cell viability (MTT assay) were determined using dose range of 3µg/ml- 192µg/ml of TCA. Based on these experiments, 3T3-L1 mouse preadipocytes and adipocytes were pretreated for 4hrs with or without 18µg/ml or 36µg/ml of TCA followed by 18hrs incubation with 200ng/ml of lipopolysaccharide (LPS). Concentration of secreted adipokines in the cell culture media and mRNA expressions were conducted using ELISA and real time PCR, respectively. Western blot analysis was conducted to determine activation of the NF-kB pathway.
Results: Compared to the control group, in the rat serum samples, there were no significant differences between the groups for the MCP-1, IL-6 and IL-10 concentrations. However, IL-6 mRNA expression in adipose tissue was significantly (p<0.05) reduced by the TCA supplementation. In both cultured 3T3-L1 pre-adipocytes and adipocytes, TCAs increased cell viability and significantly (p<0.05) reduced LPS-induced IL-6, tumor necrosis factor alpha (TNFα), and toll-like receptors 3 and 4 (TLR-3 and TLR-4) mRNA expressions. The anti-inflammatory effects of TCA were mediated, in part, by reduction in NF-kB activity as indicated by decreased LPS-induced p65 phosphorylation in TCA treated cells compared to control cells.
Conclusion: Tart cherry anthocyanins exert anti-inflammatory effects in rat adipose tissue and cultured adipocytes, which was mediated, in part by a reduction in NF-kB/p65 activity. Further clinical studies are warranted to test the efficacy of tart cherry supplementation in modulating adipose tissue inflammation in humans.
Graduate Research Assisstant
Texas Tech University