Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 286
The objective of this study was to investigate anti-inflammatory effect of berry volatiles and phenolics using a lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cell model by measuring the modulation of production of nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). We hypothesized that berry volatiles have comparable anti-inflammatory effects as berry phenolics, modulating cytokines and pro-inflammatory factors.
RAW264.7 cells were pretreated with 3 different dilutions (diluted 50, 100, and 200-fold) of six berry (blackberry, black raspberry, blueberry, cranberry, red raspberry, and strawberry) phenolic or volatile fractions for 1 hour. Cells were then incubated with or without LPS (100ng/ml) for 24 hours. The NO production was measured by using Griess reaction. The level of IL-6 and TNF-α in the culture media were quantified using ELISA kits. All data analysis was performed using Statistical Analysis System (SAS, 9.4) to determine the statistical significance, considering significant if p<0.05. Data were presented as mean ± standard error of the mean (SEM).
Results showed that berry phenolics and volatiles significantly decreased the LPS-induced NO production by 38-93% and 47-79%, respectively (P<0.01). There were no significant differences among three different dilutions of berry phenolics and volatiles in the RAW264.7 cells. Except black raspberry, berry volatiles decreased the level of TNF-α and IL-6 in LPS-stimulated RAW264.7 cells, comparable to berry phenolics.
Results from this study suggest that volatiles from six common berries have comparable anti-inflammatory activity as phenolics in vitro.
University of Arkansas