Poster Topical Area: Maternal, Perinatal and Pediatric Nutrition

Location: Hall D

Poster Board Number: 408

P13-151 - Effects of Dietary Oligofructose Alone or in Combination with 2´Fucosyllactose on the Composition of Gut Microbiota in Neonatal Piglets

Sunday, Jun 10
8:00 AM – 6:00 PM

Objectives: Human milk contains a complex mixture of oligosaccharides (HMO). Prebiotics are known to modulate gut micorbiota composition, while HMO are hypothesized to exert more specific effects. Herein, the impact of dietary 2′FL, a predominant HMO, in combination with prebiotic oligofructose (OF) on the neonatal pig gut microbiota composition was assessed.

Methods: Two-day-old piglets (n = 33) were randomized to three diets: control (CON) [0 g/L OF + 0 g/L 2′FL, Purina ProNurse], OF [CON + 5 g/L OF], or OF+2′FL [CON + 5 g/L OF + 1. 0 g/L 2′FL]. On postnatal day 34, ascending colon (AC) contents and feces were collected and gut microbiota were assessed by 16S rRNA gene sequencing and real-time PCR. The overall composition of microbiota was analyzed by principal co-ordinate analysis (PCoA) and distance-based redundancy analysis (db-RDA). The relative abundances of bacterial genera and real-time PCR data were compared by one-way ANOVA.


Results:
The overall microbiota of CON differed from OF and OF+2′FL in both AC and feces (PCoA and dbRDA, P < 0. 05). In AC, CON had higher relative abundances of Lactococcus and Streptococcus, lower proportions of Blautia, Acidominococcus and Megasphaera compared to OF and OF+2′FL. In feces, proportions of Bacteroides and Oscillospira were higher, whereas Acidaminococcus and Megasphaera were lower in CON than OF and OF+2′FL. OF and OF+2′FL piglets had similar overall microbial composition in both AC and feces (P = 0. 98, P = 0. 99 respectively). However, OF+2′FL piglets harbored lower (P < 0. 05) abundances of Anaerotruncus and Butyricicoccus in feces than OF piglets. Real-time PCR showed that OF and OF+2′FL lead to higher numbers of Bifidobacterium and lower prevalence of Bacteroides fragilis in feces compared to CON. In AC, abundances of Bifidobacterium were lowest in CON, intermediate in OF and highest in OF+2′FL (P< 0. 05).

Conclusions: A specific effect of 2′FL to increase the proportion of Bifidobacterium was observed in AC, while it had a minor effect on overall microbiota composition. Supplementation of OF or OF+2′FL to CON diet modulated the overall microbiota composition of AC and feces of piglets.




Funding Source: Funded by Nestlé Nutrition R&D

CoAuthors: Marcia Monaco – University of Illinois; Jian Yan – Nestlé Nutrition R&D; Ryan Dilger – University of Illinois; Sharon Donovan – University of Illinois

Mei Wang

Research Specialist
University of Illinois
Urbana, Illinois