Poster Topical Area: Vitamins and Minerals

Location: Hall D

Poster Board Number: 513

P26-052 - Diets with imbalanced levels of folic acid and vitamin B12 are related to oxidative stress and fatty acid profile in mothers and offspring

Sunday, Jun 10
8:00 AM – 6:00 PM

Objectives: The objective of this study was to determine oxidative stress markers (OS) and fatty acid profile (FAP) in mothers and offspring mice treated with diets having high folic acid (FA) and low vitamin B12 levels. It has been suggested that unbalanced FA/B12 ratio in pregnant mice may induce OS and disrupt fatty acid metabolism. Folic acid and B12 are essential during gestation; however, it is likely that food fortification plus additional supplementation with FA have raised FA/B12 ratio in pregnant women, with unsuspected long term consequences in offspring.


Methods:
Female mice (C57BL/6) were fed diets containing normal FA and B12 (GC) or altered FA/B12 levels (GT, ­4x FA and ¯5x B12, Research Diets) before and during pregnancy. Placentas were obtained by cesarean (day 19; n = 13) and offspring of additional mothers (n=8) were evaluated at 60 days old. TBARS (as MDA), oxidized proteins (carbonyls), glutathione (total, GSH, and GSSG) and FAP were determined. Homocysteine (Hcy) levels were measured in plasma of mothers and offspring.


Results:
At weaning (21 days) offspring weight was higher in GT than in GC (male: 13±3 vs 8±1 g; p=0.019; female: 12±2 vs 9±2; p=0.0227). MDA levels in GT were higher both, in placentas and liver of mothers (p=0.0082) along with higher Hcy levels in plasma of mothers (p=0.0335). In liver of GT male offspring, total GSH and GSSG were higher than GC (p=0.0184 and p=0.0133, respectively). In livers of GT female offspring, MDA and carbonyls were lower than GC (p=0.0138 and p=0. 0142, respectively), along with higher values in total and reduced GSH (p=0.0068 and p=0.0100, respectively). In liver of GT mothers, saturated, monounsaturated and polyunsaturated fatty acid (SFA, MFA, PFA, p=0.0281) and DHA+EPA/linolenic acid ratio were higher (p=0.0446) and n6/n3 was lower (p=0.0281). In liver of female GT offspring SFA, MFA, PFA and the n6/n3 ratio were higher (p=0.0026), but the DHA+EPA/linolenic acid was lower (p=0.0009) than GC.


Conclusions:
Maternal and offspring's diets with high FA/B12 ratio: a) increased oxidative stress and Hcy in mothers, without effect in offspring; b) decreased total fatty acid content in liver of mothers; c) a lower ability to synthesize long-chain fatty acids in the liver of female offspring.




Funding Source: Fondecyt # 1130188 to AMR.
Stekel fellowship to EC
CONICYT-PCHA/Doctorado Nacional 2017-21170259

CoAuthors: Erika CastaƱo-Moreno, MSc – University of Chile; Miguel Llanos – University of Chile

Ana M. Ronco

Professor
University of chile
Santiago, Region Metropolitana, Chile