Poster Topical Area: Maternal, Perinatal and Pediatric Nutrition

Location: Hall D

Poster Board Number: 407

P13-150 - Effects of Dietary Milk Oligosaccharides on the Gut Microbiota Composition in Neonatal Piglets

Sunday, Jun 10
8:00 AM – 6:00 PM

Objectives: Milk is a source of glycans that shape the newborn's gut microbiome. Bovine milk-derived oligosaccharides (BMOS) contain some oligosaccharides structurally identical to the ones of human milk, but lack most that are specific for humans (HMOs). Herein, the effect of BMOS, HMOs [2'fucosyllactose (2'FL) + lacto-N-neotetraose (LNnT)], and a combination of BMOS and HMOs on piglet gut microbiota composition was assessed.


Methods:
Two-day-old piglets (n = 44) were randomized to four diets: control (CON; Purina ProNurse), BMOS (CON + 6. 5 g/L BMOS), HMOs (CON + 1. 0 g/L 2´FL + 0. 5 g/L LNnT), or HMOs+BMOS (CON + 6. 5 g/L BMOS + 1. 0 g/L 2´FL + 0. 5 g/L LNnT). On postnatal day 34, ascending colon (AC) contents and feces were collected and gut microbiota was assessed by 16S rRNA sequencing. Microbiota composition was analyzed by distance-based redundancy analysis (db-RDA) and the relative abundances of bacterial genera were compared by 2-way ANOVA.


Results:
BMOS and BMOS+HMOs affected the overall microbiota composition in feces (db-RDA, P = 0. 008). Relative abundances of Escherichia, Megasphaera and Acidaminococcus were higher and Oscillospira and Ruminococcus were lower (P < 0. 05) in feces of piglets fed BMOS-containing diets. ). In AC, BMOS-containing diets (BMOS, HMOs+BMOS) lowered abundances of Dorea, Eubacterium, Peptococcus, Lactococcus and Desulfovibrio compared to piglets consumed diet without BMOS. Diets with HMOs did not affect overall bacterial communities, but decreased the proportions of Coprococcus in AC regardless of presence of BMOS. In AC, Blautia was higher (P < 0. 05) in HMOs than all other groups. Fecal Bacteroides were highest in HMOs+BMOS and lowest in HMOs.


Conclusions:
Supplementation with 6.5 g/L BMOS alone or in combination with HMOs affected overall fecal microbiota composition in the piglet model, whereas 1.5 g/L HMOs alone increased the relative proportion of specific taxa like Blautia, the 2nd most abundant genus. Ongoing analyses are exploring possible associations with phenotypes.




Funding Source:

Funded by Nestlé Nutrition R&D.

CoAuthors: Marcia Monaco – University of Illinois; Jian Yan – Nestle Nutrition R&D; Ryan Dilger – University of Illinois; Sharon Donovan – University of Illinois

Mei Wang

Research Specialist
University of Illinois
Urbana, Illinois