Poster Topical Area: Global Nutrition

Location: Hall D

Poster Board Number: 556

P12-021 - Carioca beans (Phaseolus vulgaris) prebiotic extracts may improve the gut microbiota and iron related protein expression in vivo (Gallus gallus)

Sunday, Jun 10
8:00 AM – 6:00 PM

Iron deficiency is the most common micronutrient deficiency worldwide. Biofortification of staple food crops (as common beans) aims to reduce this deficiency. However, legumes carry both inhibitors of iron absorption, such as phytic acid and certain polyphenols. Beans also contain prebiotic compounds that may affect iron absorption via intestinal bacterial population’s activity that may lead to a more acidic luminal pH, that may result in an increased iron solubility and therefore absorption. By using the Gallus gallus model and the intra-amniotic administration procedure, we aimed to investigate the effect of prebiotic extracts from various bean varieties on the intestinal brush border membrane iron related proteins, and intestinal bacterial populations. Eight treatment groups [(non-injected; 18WH2O; 40mg/mL Inulin; 50 mg/mL BRS Perola (carioca standard); 50 mg/mL BRS Cometa (carioca, Fe biofortified); 50 mg/mL BRS Esteio (black, standard); 50 mg/mL SMN 39 (black, Fe biofortified); 50 mg/mL BRS Artico (white, standard)] were utilized. Upon hatch, blood, cecum, small intestine and liver were collected for assessment of serum and liver iron concentration, the relative abundance of intestinal microbiota and expression of Fe-related and brush border membrane functional genes. Also, the polyphenol profile was analyzed from the beans flours by Ultra Performed Liquid Chromatography – tandem mass spectrometer. Carioca bean flours presented higher (p> 0.05) concentrations of kaempferol 3-glucoside, catechin and epicatechin compared to the white and black bean flours. Whereas, the black bean flours presented higher (p> 0.05) concentration of quercetin 3-glucoside, myricetin 3-glucoside and quercetin. Several treatment groups prebiotic extracted reduced presented increased relative abundance of Bifidobacteria and Lactobacilli in comparison to controls (non injected and H2O). The expression of hepcidin was higher (p<0.05) in the BRS Esteio group and lower (p<0.05) in the BRS Cometa group. The relative expression of ZnT1, ferroportin and AP were up-regulated BRS Cometa group (Fe biofortified carioca beans). The prebiotic extracts from carioca beans may improve the iron bioavailability by affecting intestinal bacterial populations and up-regulating the BBM functional proteins.


CoAuthors: Nikolai Kolba – USDA-ARS; Jon Hart – USDA-ARS; Hercia Martino – UFV ; Marilia Nutti – Embrapa; Raymond Glahn – USDA-ARS; Elad Tako – USDA-ARS

Desirre M. Dias


USDA-ARS
Ithaca, New York