Poster Topical Area: Obesity

Location: Hall D

Poster Board Number: 723

P23-096 - Effects of dietary fatty acids with 18 carbons on metabolic and inflammatory response to lipopolysaccharide treatment

Sunday, Jun 10
8:00 AM – 6:00 PM

Objectives: The objective of this study was to determine effects of dietary fatty acids with different degrees of saturation on LPS-induced inflammation and metabolic/thermogenic markers in vivo.

Methods: Male C57BL/6 mice were fed a control diet containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil (CON) or high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from shea butter (saturated fatty acid-rich fat; SHB), olive oil (n-9 monounsaturated fatty acid-rich oil; OO), or soybean oil (n-6 polyunsaturated fatty acid-rich oil; SBO) ad libitum for 4 weeks with or without a terminal 4-hour lipopolysaccharide (LPS) treatment. Gene expression was determined by quantitative Real-time PCR.

Results: Compared to CON, HFD-fed mice had higher weight gain (pp=0.0058). Mice fed OO diet had the highest BAT mass (p=0.0400) and hypothalamic Lepr expression (p=0.0045) while those fed SBO diet had higher Il6 and lower Cpt1a expression in subcutaneous WAT than other HFD groups (p=0.0629, p=0.0316, respectively). Injection of LPS significantly upregulated Il6, Mcp1, and Tnfa expression in BAT, WAT, liver, skeletal muscle, and hypothalamus, and induced higher glucose:insulin ratio (pUcp1 expression in subcutaneous WAT (p=0.0930), and significantly downregulated Pgc1a expression in both subcutaneous and epididymal WAT (p=0.0053, p=0.0003, respectively) and Cpt2 expression in the liver (p=0.0196).

Conclusions: These data indicate that LPS-induced inflammation may suppress thermogenesis and reduce insulin sensitivity. The higher expression of Il6 in subcutaneous WAT with SBO consumption and LPS injection indicate that excessive SBO consumption may worsen inflammatory response to LPS.

Funding Source: Purdue University

CoAuthors: Kolapo Ajuwon, Ph.D. – Purdue University

Sunhye Shin

Graduate Student
Purdue University
West Lafayette, Indiana