Poster Topical Area: Dietary Bioactive Components

Location: Hall D

Poster Board Number: 329

P08-071 - Extract from Hawthorn fruit Induces Cell Cycle Arrest and Apoptosis in Human Colon Cancer Cells

Monday, Jun 11
8:00 AM – 3:00 PM

Crataegus pinnatifida, commonly named hawthorn, has been used in China and other Asian countries for more than 2,000 years, both as a traditional medicinal herb and as a food component. The objective of the study was to investigate the anti-cancer activities of extract from Crataegus pinnatifid (ECP) and related molecular mechanisms.
Methods: The ground hawthorn was subjected to distilled water extraction, followed by 70%-85% ethanol precipitation, and lyophilization. Human colon cancer cell (HCT116) was treated with ECP at concentrations of 125, 250, 500, 1000 μg/ml for 12 h. Phospho-AKT and Ki-67 expression were assessed by immunofluorescence staining. Cell cycle and apoptosis were analyzed by flow cytometry. mRNA expression was examined using real-time RT-PCR.
Results: Ki-67 protein staining was reduced by ECP treatment in cells, and Phospho-AKT protein expression was significantly reduced with the ECP treatments. The proportion of apoptotic cells was significantly increased by ECP. Concomitantly, ECP treatment induced G1 phase arrest in HCT116 cells. HCT116 cells treated with 500 and 1000 μg/ml ECP had significantly increased expression of caspase 3, caspase 7, caspase 8 and caspase 9. ECP also upregulated the mRNA level of FADD, TRADD and TNFR1 in a dose-dependent manner. ECP downregulated the CyclinD1 mRNA expression.
Conclusions: Our findings demonstrated that ECP exhibited anti-cancer activities in HCT116 cells by induction of apoptosis pathway. ECP treatment also decreased proliferation by induction of G1 phase cell cycle arrest. The results indicate some potential benefits of using hawthorn extracts as functional foods for cancer patients. Further mechanistic studies are warranted to establish molecular pathways that are involved in the anti-cancer effects of ECP.


Funding Source:

USDA Cooperative State Research, Education and Extension Service, Hatch projects # ILLU-698-394 and ILLU-698-379


Immunofluorescence staining

Cell apoptosis

Cell cycle

mRNA expression

mRNA expression

CoAuthors: Guanying Xu РUniversity of Illinois at Urbana-¬Champaign; Xinyu Tang РUniversity of Illinois at Urbana-Champaign; Mingzhu Cai РUniversity of Illinois at Urbana-Champaign; Hong Chen РUniversity of Illinois at Urbana-Champaign

Lei Ma


UIUC
Urbana, Illinois