Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 335
Objective: High-density lipoprotein (HDL) particles protect from atherosclerosis via reverse cholesterol transport, as well as through antioxidant and anti-inflammatory properties. However, HDL function is impaired under chronic inflammation, as seen in metabolic syndrome (MetS). We evaluated the effects of grape ingestion on HDL functional measures in adults with MetS.
Methods: Twenty adults with MetS were asked to consume either 60 g/d of freeze-dried grape powder (GRAPE), equivalent to 2.5 cups of fresh grapes daily, or a placebo for 4-wk in a randomized, double-blind, crossover design study, separated by a 3-wk washout period. At the end of each period, fasting blood was collected for analysis of plasma lipids, HDL size and subfractions, apolipoprotein A-I (apoA-I), serum amyloid A (SAA), metabolic markers, HDL cholesterol efflux capacity, and serum paraoxonase-1 (PON1) activities. We conducted analyses on the effects of GRAPE vs. placebo in all subjects, as well as subgroup analyses in those below or above median values for HDL-cholesterol (40 mg/dL, High HDL) and HDL cholesterol efflux capacity (Low Efflux or High Efflux).
Results: GRAPE consumption did not significantly alter metabolic markers, HDL particle concentrations, HDL size, apoA-I, or PON1 activities compared with placebo in all subjects. However, in subjects with Low HDL, GRAPE resulted in lower plasma total cholesterol/HDL ratio (p = 0.04) and triglycerides (p = 0.04) compared with placebo. Compared with Low HDL, those with High HDL actually had increased markers of pro-inflammatory HDL across both intervention arms. Subjects with High HDL had improved HDL cholesterol efflux capacity (p = 0.04) and PON1-lactonase/HDL-SAA ratios (p = 0.06) with GRAPE. In those individuals with Low Efflux, GRAPE resulted in greater HDL cholesterol efflux capacity (p = 0.03) and PON1/HDL-SAA ratios (p = 0.03) compared to placebo. Overall, HDL functional measures were positively correlated with each other, and inversely correlated with measures of insulin resistance and inflammation.
Conclusions: Grape consumption may improve plasma lipids, HDL cholesterol efflux capacity, and HDL anti-inflammatory potential in distinct subsets of MetS.
University of Connecticut