Poster Topical Area: Climate/Environment, Health, Agriculture and Improved Nutrition

Location: Auditorium

Poster Board Number: 5

E17-05 - A standardized approach for comprehensive chemical profiling of foods as a basis for comparison and characterization of variance

Monday, Jun 11
8:00 AM – 3:00 PM

Objective: Develop a systematic approach to characterizing the chemical composition of foods that is fast, comprehensive, and provides expanded data on thousands of compounds.

Background: Foods consist of thousands of compounds, each with the potential to impact human health. Classical nutrient analyses are based on targeted methods that focus on a relatively few compounds and limit our view of other health-promoting possibilities and their synergism. In addition, methods for classical nutrients are time consuming and expensive and negate broad application to many foods and many components. Conversely, comprehensive chemical profiles based on non-targeted methods such as spectral fingerprinting offer hundreds to thousands of components for comparison of foods or judging their natural biological variation. Equally important, fingerprinting methods, even using high resolution mass spectrometry, require less that 5 minutes per sample and are highly cost effective.


Methods:
Fingerprinting, based on flow injection high resolution mass spectrometry (FIHRMS), combined with pattern recognition methods, such as principal component analysis (PCA), emphasizes the relationship between components and their variation. While differences between similar foods can be identified by specific ions, the identities of the compounds are not known. However, representative foods can be selected based on their fingerprints and subjected to metabolomic analysis to identify specific compounds. Initial investment in the development of a metabolite library can then be coupled with high throughput FIHRMS to rapidly identify compounds in foods and their variation as a function of cultivar, environment, and processing conditions.


Results:
Method discriminated between dry beans of different gene pools, different genotypes, grown in different states, and under normal and drought conditions and identified compounds changing under these conditions.


Conclusions:
Rapid and comprehensive analysis of foods offer greater insight into the relation of foods, diet, and health.




Funding Source:

Office of Dietary Supplements, National Institutes of Health

CoAuthors: Ping Geng – USDA; jianghao sun – USDA; Pei Chen – USDA

James Harnly

research leader
US department of agriculture
Beltsville, Maryland