Poster Topical Area: Dietary Bioactive Components

Location: Hall D

Poster Board Number: 326

P08-068 - Effects of ageLOC Defense Formula on Immune Regulation and Detoxification: Phenotype and Molecular Evaluation

Monday, Jun 11
8:00 AM – 3:00 PM

By using of a high-throughput gene expression and epigenetics based product screening platform and phenotype substantiation, we found several ingredients to have cellular protection and anti-aging characteristics. We compose an anti-aging formula: ageLOC defense formula (ADF) that included Ganoderma lucidum (Reishi), broccoli seed and Maitake extracts. An aged mice model was used to evaluate cellular protection effects of this formula. Mice at 18-m old were randomly assigned to aged control (AC, n=17) and ADF (n=16) groups. Mice at 16-wk old was included as the young controls (YC, n=17). ADF was administrated at 264 mg/kg body weight to the mice for 8-wk at ADF group. Biomarkers on immune regulatory, inflammation, and detoxification were detected in blood and liver. Gene expressions in liver and lung were also detected. As results, Nrf2 protein was significantly deceased (p<0.05) in liver after ADF and YC groups as compared to AC. Significant increases in IL-13 was observed in both ADF and YC (p<0.05), respectively. Gene expression data were analyzed by KEGG and IPA. ADF vs AC and AC vs YC were compared. In liver, reversed regulations in PPAR signaling and arachidonic acid metabolism pathways were found in two comparisons. After ADF consumption, LPS/IL-1 Mediated Inhibition of RXR Function pathway was activated as well as activated in AC vs YC; upstream regulators INFγ was activated as well as INFaR1 and PPARa were inactivated in ADF vs AC. In lung, cytokine-cytokine receptor interaction, arachidonic acid metabolism, and asthma pathways were reversely regulated in ADF vs AC as in AC vs YC. After ADF consumption as compared to AC, Upstream regulators PPARg and IL-10 were inactivated. Regulators effects analysis revealed that the inactivation of IL-10 may cause activations of inflammation responds, cell movement of blood cell, and chemotaxis. According to the results above, 8-wk ageLOC defense formula consumption by aged mice may regulate immune response and detoxification via Nrf2 protein and TH2 cytokine IL-13 phenotypically and regulate PPARγ signaling pathway in molecular level.

Liver Nrf2 protein significant decreased in in young controls and ageLOC Defense as compared to aged controls

Significant increases in IL-13 in Young controls and ageLOC defense as compared to Aged controls

Regulation on upstream regulators after ageLOC defense consumption in liver

Regulation on upstream regulators after ageLOC defense consumption in lung

Regulators effects analysis after ageLOC defense consumption in lung

CoAuthors: Yiping Ren Р1. Nu Skin (China) Daily-use & Health Products Co.LTD. Center of Anti-aging Research, Shanghai, Nu Skin Enterprises, Shanghai, China, People’s republic of; Doug Stevenson Р2. Nu Skin Enterprises, Center of Anti-aging Research, Provo, UT; Mark Bartlett Р2. Nu Skin Enterprises, Center of Anti-aging Research, Provo UT

Jihong Lu

Scientist SR. Associate
Shanghai, Shanghai, China (People's Republic)