Poster Topical Area: Dietary Bioactive Components
Location: Hall D
Poster Board Number: 318
Objective: Peroxisome-proliferator-activated receptor gamma co-activator-1 alpha (PGC-1α) play an important role in the maintenance of lipid, glucose, and energy homeostasis, and thus are related with metabolic diseases such as obesity and diabetes. This study was performed to investigate the anti-obesity effect of garlic powder in transgenic mice (TG) of PGC-1α-luciferase.
Methods: To examine the control function of PGC-1α, we generated the TG with a PGC-1α promoter (-970/+412 bp) containing luciferase as a reporter gene. PGC-1α-luciferase TG was fed a 45% high-fat diet for 8 weeks to induce obesity. Subsequently, mice were maintained on either a high-fat control diet (TG-CON), or high-fat diets supplemented with 2% (TG-GP2) or 5% (TG-GP5) garlic powder for a further 8 weeks.
Results: Dietary garlic powder reduced body weight in the TG-GL2 and TG-GL5 groups, compared to the TG-CON group. Furthermore, garlic supplementation significantly decreased plasma levels of triglycerides, total cholesterol, and leptin in the TG-GL5 groups, compared to the TG-CON group. Specifically, luciferase activity in metabolic tissues such as liver, white adipose tissue (WAT) and brown adipose tissue (BAT) was increased by garlic supplementation in a dose-dependent manner.
Conclusions: These results suggest that the anti-obesity effect of garlic powder was associated with the increase of PGC-1α luciferase activity in liver, WAT, and BAT of PGC-1α-luciferase TG. Therefore, this TG may be useful in evaluating the anti-obesity efficacy of various health functional foods.
Department of Nutritional Science and Food Management, Ewha Womans University
Seoul, Seoul-t'ukpyolsi, Republic of Korea