Poster Topical Area: Experimental Animal Nutrition

Location: Hall D

Poster Board Number: 624

P11-012 - Effects of methyl donor supplementation on embryonic malnutrition-induced low birth weight rats

Monday, Jun 11
8:00 AM – 3:00 PM

[Background and Aims] It has been reported that more than 25% of 20th and 14% of 30th women are lean in Japan. Moreover, the average birth weight is decreasing and the incidence of low birth weight delivery is increasing from 1980th to the present. According to the DOHaD theory, low birth weight is reportedly related to an increased prevalence of non-communicable disease later in life. We have established an embryonic malnutrition low birth weight rat model and found that the DOHaD model rat showed sustained high blood corticosterone concentration after restraining stress loading due to the loss of glucocorticoid action. We also found that a decrease in methionine and S-adenosylmethionine (a methyl donor of DNA and protein). We examined whether the blood concentration of corticosterone was normalized by supplementation with methyl donor supplementation. [Methods] Pups were obtained by supplementation with a methyl donor diet (D15090803, Research Diet) from the day of birth to the mother rats who fed a calorie-restricted diet (D08021202, Research Diet) during pregnancy. At the age of 7 weeks, rats were exposed with restraint stress, immediately after decapitation blood was taken, blood corticosterone concentration was measured. [Results] Blood corticosterone concentration after restraint stress decreased to the control rat level with supplementation of methyl donor diet to nursing mother. On the other hand, this effect was not observed in the feed of methyl donor diet to infant rats after weaning. [Conclusion] It was found that the blood corticosterone concentration after stress was normalized by supplementation with methyl donor via breast milk immediately after birth.

Funding Source:

Grants-in-Aid for Scientific Research (KAKENHI (Multi-year Fund)) from the Japan Society for the Promotion of Science (JSPS)

CoAuthors: Yoshihiko Kakinuma, MD – Nippon Medical School

Takahiro Nemoto

Associate Professor
Nippon Medical School
Tokyo, Tokyo, Japan