Poster Topical Area: Energy and Macronutrient Metabolism

Location: Hall D

Poster Board Number: 475

P10-075 - High-fat diet containing different DHA/EPA ratio alters fatty acid composition and attenuates hepatic lipid accumulation in ApoE-/- mice

Monday, Jun 11
8:00 AM – 3:00 PM

Objective: We have reported that dietary various ratios of docosahexaenoic acid (DHA) / eicosapentaenoic acid (EPA) have protective effects on high-fat diet (HFD) induced liver damage in mice. However, it is unclear if the protective roles are associated with the alteration of hepatic fatty acid composition in mice.


Methods: Using high-fat diet-fed ApoE-/- mice as the animal model, diets of various ratios of DHA/EPA (2:1, 1:1, and 1:2) with an ω6/ω3 ratio of 4:1 were prepared using fish and algae oils enriched in DHA and/or EPA and sunflower seed oils to partially instead of the high-fat diet.


Results: DHA/EPA supplementation significantly decreased hepatic lipid deposition, serum lipid profile, inflammatory reactions, lipid peroxidation, and expression of adipogenesis- related proteins and inflammatory factors as compared to those in the HFD group. In the varying ratios of DHA/EPA groups we found an increase in total saturated fatty acids (SFAs), total polyunsaturated fatty acid (PUFAs) ω6 series and PUFAs ω3 series in the liver compared to the HFD group. Also, the amount of monounsaturated fatty acids (MUFAs) and the ratio of ω6/ω3 showed remarkably decrease after DHA/EPA supplementation. Although, the DHA/EPA 2:1 group showed lower serum triglycerides (TG), total cholesterol (TC), low-density lipoprotein-cholesterol, IL-1β, AST, ALT, and AKP levels, and higher serum adiponectin level with the three ratio. No significant differences were found in hepatic inflammatory factors expression and hepatic lipid metabolic-related genes expression in the three DHA/EPA groups. And no significant differences within the three DHA/EPA ratio groups were found for SFAs, MUFAs, PUFAs, and ω6/ω3 ratio.


Conclusions: Our data demonstrate that dietary DHA/EPA alters hepatic fatty acids composition and attenuates HFD induced hepatic lipid accumulation, although no significant differences were found in hepatic fatty acids composition, inflammatory factors expression, hepatic lipid metabolic-related genes expression in the three DHA/EPA groups<./p>

CoAuthors: Zhiyong Gong – Wuhan Polytechnic University; Guoxun Chen – University of Tennessee at Knoxville

Liang Liu

Wuhan Polytechnic University
Wuhan, Hubei, China (People's Republic)