Poster Topical Area: Maternal, Perinatal and Pediatric Nutrition

Location: Hall D

Poster Board Number: 290

P13-032 - Colonic and Fecal Metabolites in Piglets Fed Formula Containing Sialyllactose.

Sunday, Jun 10
8:00 AM – 6:00 PM

Objectives: The gut microbiota is a highly metabolically active community of microorganisms. The siallylated milk oligosaccharides are metabolized by the infant microbiota. Herein, the effect of dietary siallylactose (SL) on metabolite profiles in the colon and feces of piglets was investigated.

Methods: Two-day-old piglets (n=34) were fed formulas containing PDX/GOS (2 g/L, each) supplemented with 0 (CON) or 380 mg/L SL, added as a SL-enriched bovine whey (Lacprodan SAL-10®, Arla Foods Ingredients Group P/S, Aarhus, Denmark), for 22 days. AC contents and feces were collected for analysis using ultra high-performance liquid chromatography/tandem accurate mass spectrometry methods (Metabolon, Durham, NC). Metabolite concentrations were compared between region (AC, feces) and by dietary SL by two-way ANOVA.

A total of 685 named biochemical were detected in AC contents and feces. There were significant main effects of region (585 compounds), diet (16 compounds) and diet x region (12 compounds). In terms of region, the majority of metabolites were higher (p<0.05) in AC compared to feces, regardless of diet and AC and feces were clearly distinguishable by principle component analysis. Comparing the same diets between AC and feces, amino acids and carbohydrates were the main differentiating compounds for the SL diet. Vitamins/cofactors, amino acids and carbohydrates were the top differentiating biochemicals for the CON diet between AC and feces. In the AC, the SL diet resulted in higher (p<0.05) levels of fructose, but lower (p<0.05) levels of caproate, heptanoate and N-acetylglucosaminylasparagine than CON. In the feces, sedoheptulose, maltose and fructose were all higher (p<0.05) in SL samples than CON. Lastly, diet altered primary and secondary bile acids; SL piglets had higher (p<0.05) glycocholate in the AC and lower (p<0.05) 3b-hydroxy-5-cholenoic acid in both the AC and feces.

Metabolomics can identify subtle metabolic variations induced by diet and variations of the gut microbiota. Herein, distinct metabolite differences between piglet AC contents and feces were apparent. Dietary SL produced differences in carbohydrates, medium chain fatty acids and bile acids, which may be related to differences in microbiota composition.

Funding Source:

Funded by Mead Johnson Nutrition

CoAuthors: Mei Wang, PhD – University of Illinois; Marcia Monaco, PhD – University of Illinois; Brian Berg, PhD – Mead Johnson Pediatric Nutrition Institute; Maciej Chichlowski, PhD – Mead Johnson Pediatric Nutrition Institute; Ryan Dilger, PhD – University of Illinois

Sharon M. Donovan

University of Illinois, Urbana
Urbana, Illinois