Poster Topical Area: Obesity
Location: Hall D
Poster Board Number: 626
Sweeteners are linked to different health effects in part due to changes in gut microbiota.
Objective: The purpose of the present work was to study the effect of different type of sweeteners on intestinal microbiota.
Methods: Wistar rats were fed different sweeteners (10%) in drinking water unless otherwise indicated with a control diet (AIN 93) for a period of 4 months (sucrose, fructose, glucose, steviol glucosides (2.5%), unrefined sugar, honey, svetia® and sucralose (1.5%) ). Body weight, food intake, body composition(NMR), biochemical parameters, glucose tolerance test, energy expenditure, short chain fatty acids (SCFA) and gut microbiota composition by sequencing in the MiSeq platform were determined.
Results: After 4 months of consuming the different sweeteners, body mass of animals fed sucrose followed by sucralose increased by 24% and 10% respectively in comparison with the control group. These sweeteners produced glucose intolerance, metabolic inflexibility and significantly increased in serum glucose, triglycerides, total and LDL cholesterol in comparison with the control group. The groups that consumed honey, unrefined sugar and steviol glucosides showed the lowest weight gain, glucose intolerance and metabolic inflexibility and maintained serum biochemical parameters in the normal range. Consumption of sucralose produced the most gut dysbiosis, particularly a significant increase in B. fragilis, whereas rats fed honey had a significant increase in B. animalis. Rats fed steviol glucosides or svetia® increased A. muciniphila, in addition Svetia ®increased F. prausnitzii. Rats consumed glucose or fructose showed changes in the Ruminoccoccus genus. Finally, the sucrose group increased P. copri and P. diastonis associated with obesity. Sucrose and sucralose produced the highest metabolic endotoxemia mediated by lipopolysaccharide and SCFA concentration.
Conclusions: The results indicate that the consumption of honey, steviol glucosides and unrefined sugar produced the lowest metabolic endotoxemia associated with a reduced dysbiosis in gut microbiota, whereas the consumption of sucralose or sucrose induce produced the highest metabolic endotoxemia, intestinal dysbiosis and glucose intolerance.
Mexico City, Distrito Federal, Mexico