Poster Topical Area: Nutrient-Gene Interactions
Location: Hall D
Poster Board Number: 456
Objective: Knowledge of the DNA methylation pattern in the appetite /satiety pathway may promote understanding of the molecular mechanisms that determine the efficacy of the treatment for weight loss. Thus, the aim of this study was to evaluate changes in the pattern of methylation of genes related to the appetite/satiety pathway in women with severe obesity before and after Roux-en-Y Gastric Bypass (RYGB).
Methods: Ten women with severe obesity (Body mass index - BMI> 40 kg/m²) submitted to RYGB were selected. Specific array-based DNA methylation analysis was performed using the Infinium HumanMethylation 450K Beadchip technology, which evaluated the methylation pattern of the genes related to the appetite/satiety pathway before and after six months of RYGB. The methylation level of each cytosine was expressed as a beta value (fluorescence intensity ratio of methylated and unmethylated alleles) ranging from 0 (unmethylated) to 1 (fully methylated).
Results: Hypermethylation of genes related to the appetite and satiety pathway was observed. Among them, the HCRTR1 (6.7%), ADIPOR2 (5.1%) and FTO (5.2%) genes were outstanding. (p <0.05, p <0.001).
Conclusion: The study revealed that RYGB promotes significant changes in DNA methylation pattern, highlighting a hypermethylation of the HCRTR1, ADIPOR2 and FTO genes involved in the appetite/satiety pathway. This study will aid in the discovery of important biomarkers that are essential for clarifying mechanisms related to apetite/ satiety in obesity.
Master student in Nutrition and Metabolism
University of Sao Paulo (USP)
Ribeirão Preto, Sao Paulo, Brazil