Poster Topical Area: Nutrient-Gene Interactions

Location: Hall D

Poster Board Number: 456

P17-033 - Hypermethylation of genes involved in appetite/satiety control after Roux-en-Y gastric bypass

Sunday, Jun 10
8:00 AM – 6:00 PM

Objective: Knowledge of the DNA methylation pattern in the appetite /satiety pathway may promote understanding of the molecular mechanisms that determine the efficacy of the treatment for weight loss. Thus, the aim of this study was to evaluate changes in the pattern of methylation of genes related to the appetite/satiety pathway in women with severe obesity before and after Roux-en-Y Gastric Bypass (RYGB).


Methods:
Ten women with severe obesity (Body mass index - BMI> 40 kg/m²) submitted to RYGB were selected. Specific array-based DNA methylation analysis was performed using the Infinium HumanMethylation 450K Beadchip technology, which evaluated the methylation pattern of the genes related to the appetite/satiety pathway before and after six months of RYGB. The methylation level of each cytosine was expressed as a beta value (fluorescence intensity ratio of methylated and unmethylated alleles) ranging from 0 (unmethylated) to 1 (fully methylated).


Results:
Hypermethylation of genes related to the appetite and satiety pathway was observed. Among them, the HCRTR1 (6.7%), ADIPOR2 (5.1%) and FTO (5.2%) genes were outstanding. (p <0.05, p <0.001).


Conclusion:
The study revealed that RYGB promotes significant changes in DNA methylation pattern, highlighting a hypermethylation of the HCRTR1, ADIPOR2 and FTO genes involved in the appetite/satiety pathway. This study will aid in the discovery of important biomarkers that are essential for clarifying mechanisms related to apetite/ satiety in obesity.




Funding Source: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fundação de Apoio Ao Ensino, Pesquisa e Assistência (FAEPA)

CoAuthors: Carolina Nicoletti – University of Sao Paulo; Vitor Pinhanelli – University of Sao Paulo; Marcela Pinhel – University of Sao Paulo; Bruno Oliveira – University of Sao Paulo; Caroline Welendorf – University of Sao Paulo; Flavia Ferreira – University os Sao Paulo; Rayana Foglietti – University of Sao Paulo; Wilson Junior – University of Sao Paulo; Carla Nonino – University of Sao Paulo

Letícia S. Wolf

Master student in Nutrition and Metabolism
University of Sao Paulo (USP)
Ribeirão Preto, Sao Paulo, Brazil